Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

MiPschool 2007 Schroecken AT Programme

From Bioblast

MiPsociety
       
MitoGlobal
       
MitoGlobal Events
       
MiPconference
       
MiPschool 2023
       


MiPschool 2007 Schroecken AT Programme

MiPsummer Programme

12-18 July 2007, Schroecken, Vorarlberg, Austria
Lectures of the MiPsummer School on "Mitochondrial Respiratory Phyisology" will provide a balance between an introduction into the basic concepts, advanced methodological and scientific approaches, and specific applications, with a focus on mitochondrial respiratory function linked to the general theme of MiP.
For addresses of lecturers, see MiPsummer Participants.
Day 1: Mitochondrial respiratory capacity, coupling and respiratory control. Programme 1 - Abstracts 1
Day 2: Basic science and clinical reality 1. Programme 2 - Abstracts 2
Day 3: Oxygen kinetics of mitochondrial respiration and inhibition by nitric oxide. Programme 3 - Abstracts 3
Day 4: Mitochondrial membrane potential. Programme 4 - Abstracts 4
Day 5: Basic science and clinical reality 2. Programme 5 - Abstracts 5

1 - Friday, 13. July

MiPsummer Morning Lectures. Mitochondrial respiratory capacity, coupling and respiratory control - Abstracts Day 1
Chair: Steven Hand (US), Susana Cadenas (ES)
1L-01 09:00-10:00 Erich Gnaiger (Innsbruck, AT) MitoPathways and mitochondrial respiration in top gear – an introduction to the concept of the ‘Q-junction’ and respiratory control with multiple substrates.

Reading: Gnaiger E (2007) in: Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck: pp.21-37.

1L-02 10:00-11:00 Erich Gnaiger (Innsbruck, AT) P/O, respiratory and uncoupling control ratios – established concepts re-visited.

Reading: Gnaiger E (2001) Respir. Physiol. 128: 277-297.

11:00 Coffee
1L-03 11:45-12:45 Kathrin Renner (Regensburg, DE) Development of a respiratory protocol to detect mitochondrial defects under drug treatment.

Reading: Renner et al (2003) Biochim. Biophys. Acta 1642: 115-123.

13:00 Lunch
MiPsummer Afternoon Workshop: Permeabilized muscle fibers and high-resolution respirometry
1W 15:00-19:00 HĂ©lĂšne Lemieux, Erich Gnaiger (Innsbruck, AT)

Permeabilized fiber preparation: Mouse heart. Multiple substrate/inhibitor protocol: A lecture hall experiment on-line. Cytochrome c oxidase inhibitor titration – biochemical threshold and excess capacity.

17:00-18:15 Coffee and Posters
Posters:
1P-01 Marco Alves, Paulo J Oliveira, Rui A Carvalho (PT) Preservation of cardiac mitochondrial function during ischemia and ischemia-reperfusion: role of the cardioplegic protection by Celsior and Histidine buffer solution.
1P-02 Dominique-Marie Votion (BE), HĂ©lĂšne Lemieux, Ange Mouithys-Mickalad, Didier Serteyn, Erich Gnaiger. Study of the mitochondrial respiration in the equine muscle with high-resolution respirometry: feasibility, preliminary results and potential applications.
1L-04 18:15-19:00 HĂ©lĂšne Lemieux (Innsbruck, AT) High excess capacity of cytochrome c oxidase in permeabilized fibers of the mouse heart.

Reading: Lemieux H, Gnaiger E (2007) in: Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck: pp. 38-42.

20:00 Dinner at Hotel Mohnenfluh'

2 - Saturday, 14. July

MiPsummer Morning Lectures. Basic science and clinical reality
Chair: Kathrin Renner (DE), Erich Gnaiger (AT)
2L-01 09:00-10:00 Hong Kyu Lee (Seoul, KR) The metabolic syndrome and mitochondrial function - towards an international mtDNA cybrid study group.
2L-02 10:00-11:00 Jaap Keijer (Wageningen, NL) Bioactive food components and energy metabolism.
11:00 Coffee
2L-03 11:45-12:45 Vilma Borutaite (Kaunas, LT) Mitochondrial regulation of cell death: NO and cytochrome c redox state.

Reading: Mander P et al.(2005) J. Neurosci. Res. 79: 208-215.

13:00 Lunch
MiPsummer Special Topic: Basic science and clinical reality
2L-04 15:00-15:45 Hong Kyu Lee (Seoul, KR) Diabetes 2 and the role of mitochondrial pathophysiology.

Flash presentations of posters:

2P-01 15:50-16:00 Sandra Amaral, LuĂ­s Martins, Paula C. Mota, Paulo J. Oliveira, JoĂŁo Ramalho-Santos (PT) Mitochondrial testicular function: Effects of age and diabetes
2P-02 16:00-16:10 Timothy R Koves, Noland RC, Slentz DS, Ilkayeva O, Newgard CB, Muoio DM (US) Mitochondrial dysfunction and metabolic inflexibility in models of aging and diet-induced obesity.
2P-03 16:10-16:20 Joris Hoeks, JJ Briedé, J de Vogel, G Schaart, M Nabben, E Moonen-Kornips, MKC Hesselink, P Schrauwen (NL) Mitochondrial function, -content and -ROS production in rat skeletal muscle: effect of high-fat feeding.
2P-04 16:20-16:30 Maxi Meissner, G Henk Visser, Frans Stellaard, F Kuipers (NL) Bile acid signaling, FXR and energy expenditure – novel perspectives for the treatment of metabolic diseases.
16:30–19:00 Coffee and Posters
2P-05 Ruwen Böhm, L Schild, Th Herdegen (DE) Different approaches to assessing mitochondrial function and their utility and accuracy for pharmaceutical testing.
2P-06 Peter Lindinger, Martine Calame, Matthias Hoch, Alex N Eberle, Ralph Peterli, Thomas Peters, Sjouke Hoving, Hans Voshol, Ian Fearnley, John E Walker (CH) Proteome analysis of mitochondria in adipose tissues of Bariatric patients: Identification of risk factors and/or predictors for the treatment outcome.
2P-07 Alena ÄŒĂ­ĆŸkovĂĄ, StrĂĄneckĂœ V, IvĂĄnek R, HartmannovĂĄ H, NoskovĂĄ L, PiherovĂĄ L, TesaƙovĂĄ M, HansĂ­kovĂĄ H, HonzĂ­k T, Zeman J, Divina P, Paul J, HouĆĄtěk J, Kmoch S (CZ) h-MitoArray and gene expression analysis in 13 patients with mitochondrial ATP synthase deficiency.
2P-08 Daniela Fornuskova, Stiburek L, Pejznochova M, Zeman J (CZ) Depletion of COX5A subunit in HEK293 cells.
2P-09 Virginie Rhein, G Baysang, F Meier, L Ozmen, H Bluethmann, E Savaskan, F MĂŒller-Spahn, C Czech, J Götz, A Eckert (CH) Changes in mitochondrial respiration in brain cells of transgenic mice models of Alzheimer’s disease
2P-10 Olga Brantova, J Sladkova, H Hansikova, J Zeman (CZ) Fibroblasts cell culture as model for apoptosis detection.
2P-11 Karin Eberhart, Ireen Ritter, Birgit Timischl, Reinhard Kofler, Peter Oefner, Kathrin Renner (DE) Synergistic effect of glucocorticoids and 2-deoxyglucose - energy metabolism as a new target in the treatment of leukemia.
2P-12 Zdeněk Paris, Julius Lukeơ (CZ) Functional analysis of genes encoding mitochondrial proteins in Trypanosoma brucei.
20:00 Dinner at Hotel Mohnenfluh

3 - Sunday, 15. July

MiPsummer Morning Lectures: Oxygen kinetics of mitochondrial respiration and inhibition by nitric oxide
Chair: Vilma Borutaite (LT), Jaap Keijer (NL)
3L-01 09:00-10:00 Erich Gnaiger (Innsbruck, AT) Respiratory control by oxygen pressure under normoxia and hypoxia.

Reading: Gnaiger E (2003) Adv. Exp. Med. Biol. 543: 39-55.

3L-02 10:00-11:00 Alessandro Giuffre (Rome, IT) Inhibition of respiratory oxidases by NO: Mechanistic and physiological aspects.

Reading: Brunori M et al (2006) Biochim. Biophys. Acta. 1757:1144-1154.

11:00 Coffee
3L-03 11:45-12:45 Susana Cadenas (Madrid, ES) Inhibition of cellular respiration by edogenous nitric oxide and relative sensitivity of the main two cellular targets of nitric oxide.

Reading: Rodríguez-Juárez et al (2007) Biochem. J. 405: 223–231.

13:00 Lunch
MiPsummer Afternoon Workshop: Spectrophotometry and oxygen kinetics. – NO and kinetic models.
3W 15:00-17:00 Natascha Sommer(DE), Francesca Scandurra, Thomas Derfuss, Erich Gnaiger. Simultaneous remission spectrophotometry and high-resolution respirometry. Respiratory control by oxygen and redox states of mitochondrial cytochromes in cells: Instrument demonstration.
17:00-18:00 Coffee and Posters
3P-01 Enara Aguirre, F RodrĂ­guez-JuĂĄrez, E Gnaiger, S Cadenas (ES) Inhibition of cellular respiration by nitric oxide during normoxia and hypoxia.
3P-02 Felix RodrĂ­guez-JuĂĄrez, E Aguirre, S Cadenas (ES) Relative sensitivity of soluble guanylate cyclase and mitochondrial respiration to endogenous nitric oxide at physiological oxygen concentration.
3D 18:00-18:30 Discussion: Oxygen kinetics, NO and models of competitive and non-competitive inhibition.
19:00 Dinner at Hotel Mohnenfluh
20:15 Open air folk music – garden of Hotel Mohnenfluh.

4 - Monday, 16. July

MiPsummer Morning Lectures: Mitochondrial membrane potential and respiratory control.
Chair: Rodrigue Rossignol (FR), Alessandro Giuffre (IT)
4L-01 09:00-10:00 Vilma Borutaite (Kaunas, LT) Mitochondrial membrane potential and respiratory control.
4L-02 10:00-11:00 Steven C Hand (Baton Rouge, US) Mitochondrial membrane potential, pH gradients, and redox state.

Reading: Menze MA et al (2005) Am. J. Physiol. Regul. Integr. Comp. Physiol. 289: R68–R76.

11:00 Coffee and Posters
Posters:
4P-01 Magdalena Labieniec, Cezary Watala, Dominika Malinska (PL) Resorcylidene aminoguanidine (RAG) affects calcium mobilization and transmembrane potential in isolated rat liver mitochondria without impairments of respiratory chain.

Flash presentations and posters:

4P-02 11:30-11:40 Dominika MaliƄska, Adam Szewczyk (PL) Mitochondrial potassium channel opener BMS-191095l – cytoprotective properties and the influence on mitochondrial physiology.
4P-03 11:40-11:50 David Julian, Allman Rollins (US) Mitochondrial movement is by Brownian motion in coelomocytes from an annelid worm.

In case of favourable weather conditions:

12:00 MiPsummer Walk to the cheese and wine reception at the Alpmuseum uf m Tannberg; refreshment in the lake Körebersee or at Hotel Körbersee.'
18:30 MiPsummer Grill Party.
21:00 Meeting of the MiPboard – MiP2008 (round table conference), MiP2009 (joint conference with UMDF), future perspectives (open for all participants).

5 - Tuesday, 17. July

MiPsummer Morning Lectures: Basic science and clinical reality
Chair: Hong Kyu Lee (KR), Dominique-Marie Votion (BE)
5L-01 09:00-10:00 Rodrigue Rossignol (Bordeaux, FR) Mitochondrial bioenergetics and structural network organization.
5L-02 10:00-11:00 Enrico Calzia(Ulm, DE) Small and large animal models of sepsis and I/R-injury - how to combine basic science and clinical reality.
11:00 Coffee
5L-03 11:45-12:45 Charles Hoppel (Cleveland, US) Integrated mitochondrial function and the clinical utility of polarographic analysis.
13:00 Lunch
15:00 MiPsummer Afternoon Workshop: Lectures on open topics; Round table discussion; Summary - evaluation
20:00 MiPsummer Dinner at Hotel Mohnenfluh
5A 22:00 Charles Hoppel (Cleveland, US) Inner mitochondrial membrane and outer space.