MiPNet23.02 IOC127 Szeged HU
 Szeged HU, 2018 Mar 27. Oroboros satellite O2k-Workshop on hypoxia IOC127. |
Oroboros (2018-03-27) Mitochondr Physiol Network
Abstract:
Oroboros O2k-Workshop on hypoxia in cooperation with HU Szeged Boros M. Szeged, Hungary, 2018 March 27.
• O2k-Network Lab: AT_Innsbruck_OROBOROS, HU Szeged Boros M
General information
- This O2k-Workshop takes place satellite to the WISe3 Conference in Szeged, HU. Separate registration is required for this workshop.
- Three topics will be covered during the one-day workshop:
- (1) Andras Meszaros: Closed-chamber mitochondrial oxygen kinetics with demo experiment and introduction of the data analysis module O2kinetics - new development.
- (2) Erich Gnaiger: Assessment of mitochondrial and cellular oxygen kinetics by steady-state oxygen titrations, using the Titration-Injection microPump TIP2k,
- (3) Timea Komlodi: Measurement of H2O2 production rate with the O2k-FluoRespirometer and Amplex UltraRed(R) fluorescence. Dependence of ROS production on oxygen concentration.
- Three topics will be covered during the one-day workshop:
Venue
- Clinical Skills Centre, Faculty of Medicine
- University of Szeged
- 13 Dom square, HU-6720
- Szeged, Hungary
- HU Szeged Boros M
Local organizers
Prof. Boros Mihaly, HU Szeged Boros M
Univ-Doz. Dr. Andrea Szabo, HU Szeged Boros M
Registration
- The registration is free of charge, but we ask you for informal registration by Mar 19 at latest.
- The number of participants will be limited. Please let us know by email if you will attend: [email protected]
Tutors and Lecturers
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
András Mészáros, MD., PhD., Chief Research Officer, Oroboros Instruments and Assistant Professor, University of Szeged, Institute of Surgical Research
Timea Komlòdi, Scientific assistant, Oroboros Instruments
Programme
Tuesday Mar 27
| Time | Topic | Materials | |
|---|---|---|---|
| 09:00 | Morning session on mitochondria | ||
| 09:00 | 
|
Petr Pecina
Changes in oxygen kinetics between COX4i1 and COX4i2-containing COX |
|
| 09:30 | 
|
Laszlo Tretter
Bioenergetical approach of neurodegenerative diseases |
|
| 10:00 | 
|
Laszlo Juhasz
Ca(2+)N it be measured? High-resolution O2k-FluoRespirometric detection of extramitochondrial calcium movement |
|
| 10:10 | David Kurszan Jasz
Mitochondrial effects of methane gas treatment on rat cardiomyocytes subjected to simulated ischemia/reperfusion |
||
| 10:20 | Eszter Tuboly
Novel cardioprotective effects of methane in an ex vivo model |
||
| 10:30 | Coffee/tea break | ||
| 10:45 | 
|
Andras Meszaros
Mitochondrial oxygen kinetics in closed-chamber respirometers - Introduction. |
Gnaiger 1995 J Bioenerg Biomembr |
|
Mitochondrial oxygen kinetics in closed-chamber respirometers - O2k demo experiment. |
|||
|
Mitochondrial oxygen kinetics in closed-chamber respirometers - Data analysis. Introduction of the new DatLab analysis module O2kinetics |
||
| 12:15 | Lunch break | ||
| 13:00 | 
|
Chinopoulos Christos
Interplay of respiratory components and mitochondrial diaphorases on redox state in anoxia | |
| 13:30 | 
|
Timea Komlodi
Oxygen dependence of H2O2 production: an application of high-resolution respirometry with Amplex UltraRed(R) fluorescence. |
Hydrogen peroxide |
|
Oxygen dependence of H2O2 production: Data analysis | Hydrogen peroxide | |
| 14:30 | Coffee/tea break | ||
| 14:45 |  |
Erich Gnaiger
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - A comparison of steady-state and closed-chamber mitochondrial oxygen kinetics. |
TIP2k-Module |
 |
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Programming the TIP2k. |
TIP2k-Module | |
|
|
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Calculations and data analysis. Theoretical background. |
Harrison 2015 J Appl Physiol | |
| 16:15 | Coffee/tea break | ||
| 16:30 | COST Action MitoEAGLE session | ||
| 16:30 | Ledo Ana Margarida
Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer’s disease: implications on mitochondrial function |
||
| 16:45 | 
|
Krako Jakovljević Nina
Investigation of subcellular mechanisms in insulin resistance models in hepatocytes and myocytes |
|
| 17:00 | 
|
Beata Cizmarova
The effect of short-term exopsure to moderate altitude on respiration of peripheral-blood mononuclear cells |
|
| 17:15 | Poster session and discussions | ||
| Andrea Evinova
Magnesium protects from calcium induced collapse of mitochondrial trans-membrane potential. | |||
|
Kasja Pavlovic
C2C12 myoblasts as a cell model for studying the role of mitochondria in insulin resistance | ||
| Ioana Pavel
The acute and chronic effects of a benzylamide derivative of maslinic acid in liver mitochondria isolated from mice with chemically induced skin carcinogenesis |
List of participants
| Participant | Institution | |
|---|---|---|
| Bori Zoltan | University of Physical Education, Budapest | |
| Budai Andras | HU Budapest Szijjarto A - Semmelweis University | |
|
|
Chinopoulos Christos | HU Budapest Chinopoulos C - Semmelweis University |
|
Kolonics Attila | BioFirmware Ltd |
| Koltai Erika | University of Physical Education, Budapest | |
| Pavel Ioana | RO Timisoara Muntean DM - Victor Babes University of Medicine and Pharmacy | |
|
|
Pecina Petr | CZ Prague Houstek J - Academy of Sciences of the Czech Republic |
| Talas David | University of Szeged | |
| Sergeeva Tatiana | RU Nizhny Novgorod Mukhina I - Nizhny Novgorod State Medical Academy | |
|
|
Tretter Laszlo | HU Budapest Tretter L - Semmelweis University |
| Windedal Tobias |
WISe3 Conference Szeged
Contact
- Oroboros Instruments
- High-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
- Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - »Bioblast link«
- Isolated mitochondria and intact cells.
- Nanomolar resolution of oxygen concentration.
- Effects of cell density and activity.
- Closed versus open systems.
- p50 in vivo.
- Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - »Bioblast link«
- Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«
- A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen
- Mitochondrial kinetics measured by high-resolution respirometry
- Calibrations and corrections for response time and instrumental background
- Steady-state injection respirometry
- Mitochondrial respiratory control at low oxygen
- Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration
- Effect of ADP and oxygen limitation on ADP/O2 flux ratios
- The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress
- Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«
- Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - »Bioblast link«
- First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph.
- Standardized oxygen calibration (air and internal zero oxygen calibration).
- Automatic instrumental background correction of oxygen flux.
- Deconvolution of the oxygen signal for time resolution required in oxygen kinetics.
- Quality control of the limit of detection of oxygen flux at ±1 pmol O2·s-1·ml-1.
- Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - »Bioblast link«
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2018
O2k-Workshops are listed as MitoGlobal Events.'
