MiPNet22.03 IOC119 Innsbruck AT

MiPNet22.03 IOC119 Innsbruck AT

Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119.

Oroboros (2017-02-27) Mitochondr Physiol Network

Abstract:

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.

• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

Programme

Tuesday 28th	Topic	Room
17:00 - 18:00	Welcome - common aims	MiPArt

Wednesday 1st	Topic	Room
09:00	Mitochondrial and cell research	MiPArt
10:00	OroboPOS service and O2k instrumental setup - overview with videoclips	MiPArt
10:30	Hands-on: OroboPOS service and O2k instrumental setup	MiPArt
12:00	Lunch break
13:00	Instrumental quality control 1: oxygen calibration	MiPArt
14:00	Instrumental quality control 2: Instrumental O2 background	MiPArt
15:00	Coffee
15:30-18:00	Hands-on: Instrumental quality control 1 and 2. DatLab analysis.	ORO-MitoFit Lab

Thursday 2nd	Topic	Room
09:00	Introduction to SUIT protocols RP1&RP2	MiPArt
10:00	Coffee
10:30	O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.	MiPArt
12:00	Lunch break
13:00	Introduction to DatLab analysis	MiPArt
15:00	Coffee
15:30-18:00	Individual DatLab analysis + summary	ORO-MitoFit Lab

Friday 3rd	Topic	Room
09:00	SUIT protocols	MiPArt
10:30	Coffee
11:00	Q&A session on HRR and OXPHOS analysis: Design of experimental protocols	MiPArt
12:00	Lunch break
13:00	O2k-Core and O2k-Multisensor	MiPArt
14:00	DatLab analysis	ORO-MitoFit Lab
15:00	Coffee
15:30	DatLab analysis	ORO-MitoFit Lab
16:15	The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions	MiPArt
17:30	Farewell activity	MiPArt

General information

The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.

Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR).

» Information on travel and venue: IOC Innsbruck.

Registration

Fully booked.

Maximum number of 6 participants.

Tutors

Participants

	Participant	Institution
	Anja Bufe	AT Innsbruck Oroboros : Oroboros Instruments, Innsbruck (AT)
	Eugenia Carvalho	US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
	Matthew Cotter	US AR Little Rock Borsheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US)
	Ivone Leong	SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
	Yue Hugh Guan	Shanghai (CN)
	Vasco Sequeira Oliveira	NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL)
	Anna-Maria Wintler	SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE)
	Evelyn Zacharewicz	NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL)

Contact

[email protected]

Oroboros Instruments

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.

IOC recommended reading

» IOC recommended reading

» MitoPedia: Respiratory states

O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.

Labels:

HRR: Oxygraph-2k

ORO, IOC, MitoFit, TRACT, 2017