MiPNet19.05 IOC90 Bolzano IT
 Bolzano IT, 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. IOC90 at ISMM2014. |
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Oroboros (2014-05-27) Mitochondr Physiol Network
Abstract:
90th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.
• O2k-Network Lab: AT Innsbruck Oroboros
Labels:
Stress:Ischemia-reperfusion, Temperature
HRR: Oxygraph-2k
ORO, IOC, 2014, MitoCom
Conference
- ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31
O2k-Workshop
- TUESDAY 2014-05-27 / 14.00 - 17.30
- Seminar room 8
- The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H2O2 production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the Oroboros O2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.
- » More details? Consult Hypothermia-Network: Mitochondria in the Cold.
Introduction
14:00 – 14:45 Introduction: high-resolution respirometry and mt-function in extreme environments
Gnaiger Erich, CEO;
Fontana-Ayoub Mona; Biomedical assistant;
Fleischmann Sandra; Administrative assistant;
Laner Verena; Project manager and scientific assistant
- Oroboros Instruments - General introduction into the Oroboros O2k and high-resolution respirometry, O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module Titration-Injection microPump).
Demo experiment
14:45 – 15:30 Demo experiment: mitochondrial respiration and H2O2 production under hypoxia
15:30 – 16:00 Coffee break
Round table: Hypoxia and mitochondrial function - practical experience
16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oroboros O2k to >4,000 m
- Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.
- Gnaiger Erich, AT - Boushel_RC, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK
- Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.
Round table: Hypothermia and mitochondrial function - perspectives
16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function
- Keynote statements by
- Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants
- Keynote statements by
Participants
- Harald Engan (Mid Sweden University)
- Irene Pichler (EURAC Bolzano/Bozen - Center for Biomedicine)
- Lindsay Benage (Colorado State University)
- Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine)
- Mark Olfert (West Virginia University)
- Petra Platen (Prof Dr Ruhr University Bochum, Sportmedizin)
- Chris Müller (LMU München)
- Vittore Verrati (Chieti University)
- Marika Falla (Ospedale di Bolzano, Neurologia)
- Paolo Laveder (University of Padova, Italy, Dep of Biology)
- Daniel Martin (University College London)
- Edward Ojuka (University of Cape Town, South Africa)
Guest tutors and invited experts
- Robert Boushel (The Swedish School of Sport and Health Sciences)
- Adam Chicco (Colorado State University, Department of Health and Exercise Science)
- Andrew Murray (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
- James Horscroft (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
- Sandro Malacrida (EURAC Bolzano/Bozen - Center for Biomedicine)
Registration
- Visit us at the Oroboros Instruments exhibition booth from Monday to Wednesday, May 26 to 28.
- Here you can register for the Oroboros O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants.
Recommended reading
- O2k-Publications: Hypoxia
- Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3:1135-90. »Abstract
- O2k-Publications: Oxygen kinetics
- O2k-Publications: Temperature
SUIT protocols for high-resolution respirometry
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
HRR and O2k-Fluorometry
- » Manual: MiPNet17.05 O2k-Fluo LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »Open Access
- » O2k-Fluorometry
- » Manual: MiPNet17.05 O2k-Fluo LED2-Module
Mitochondrial pathways
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. » Open Access - handout to O2k-Workshop participants.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.
