Abstract: Lemieux H, Votion DM, Doerrier C, Gnaiger E (2019) Mitochondrial respiration in permeabilized fibers: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23(08):1-4. Methodological and conceptual features of High-Resolution Respirometry (HRR) are illustrated in an experiment with permeabilized fibers in the Oroboros O2k. The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibers. Application of the DatLab software is shown for real-time data analysis (compare MiPNet12.09). The following guideline describes the SUIT-011 protocol and includes a short discussion of results (see Votion 2012 PLoS One). The experiment was carried out by participants of an O2k-Workshop on HRR in December 2007 (IOC44; Schroecken, Austria).
Labels: MiParea: Respiration, Instruments;methods
Organism: Horse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue
Regulation: Coupling efficiency;uncoupling, Cyt c Coupling state: LEAK, OXPHOS, ET Pathway: N, S, NS HRR: Oxygraph-2k, O2k-Protocol
O2k-Demo, O2k-Core, SUIT-011, SUIT-011 O2 pfi D024
- SUIT protocol: SUIT-011
|Step||Respiratory state||Pathway control||ET-Complex entry into Q-junction|
Figure 1. Oxygen concentration ([μM] blue line) and oxygen flux per mg wet weight of muscle ([pmol∙s-1∙mg-1] red lines) in O2k chamber B, in permeabilized fibers from horse skeletal muscle with the standard titration protocol.
- Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. - »Bioblast link«