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Messer 2000 J Biomed Materials Res

From Bioblast
Publications in the MiPMap
Messer RLW, Doeller JE, Kraus DW, Lucas LC (2000) An investigation of fibroblast mitochondria enzyme activity and respiration in response to metallic ions released from dental alloys. J Biomed Materials Res 50:598-604.

Β» PMID: 10756319

Messer RLW, Doeller JE, Kraus DW, Lucas LC (2000) J Biomed Materials Res

Abstract: Most cellular functions evaluated for biocompatibility are high-energy processes such as proliferation and therefore are not usually affected before a decrease in energy production is observed. Several studies have shown that metabolic functions are altered at much lower concentrations than several normally used biocompatibility tests such as viability. Therefore, the purpose of this study was to provide an in-depth evaluation of metallic ion effects on mitochondria function and thereby biocompatibility. These studies evaluated the mitochondrial function of human gingival fibroblasts exposed to the salt solutions of ions released from nickel-based dental alloys, particularly beryllium (Be(2+)), chromium (Cr(6+) and Cr(3+)), nickel (Ni(2+)), and molybdenum (Mo(6+)). Mitochondrial function was examined by NADH:CoQ reductase activity, succinate dehydrogenase activity, and oxygen consumption. β€’ Keywords: Metal ions, Cytotoxicity, metabolic, Biocompatibility, Cell culture

β€’ O2k-Network Lab: US AL Birmingham Kraus DW


Organism: Human  Tissue;cell: Fibroblast  Preparation: Intact cells 

HRR: Oxygraph-2k 

Pharmacology; Biotechnology