Maurya 2018 Cell Rep
|Maurya SK, Herrera JL, Sahoo SK, Reis FCG, Vega RB, Kelly DP, Periasamy M (2018) Sarcolipin signaling promotes mitochondrial biogenesis and oxidative metabolism in skeletal muscle. Cell Rep 24:2919-31.|
Abstract: The major objective of this study was to understand the molecular basis of how sarcolipin uncoupling of SERCA regulates muscle oxidative metabolism. Using genetically engineered sarcolipin (SLN) mouse models and primary muscle cells, we demonstrate that SLN plays a crucial role in mitochondrial biogenesis and oxidative metabolism in muscle. Loss of SLN severely compromised muscle oxidative capacity without affecting fiber-type composition. Mice overexpressing SLN in fast-twitch glycolytic muscle reprogrammed mitochondrial phenotype, increasing fat utilization and protecting against high-fat diet-induced lipotoxicity. We show that SLN affects cytosolic Ca2+ transients and activates the Ca2+/calmodulin-dependent protein kinase II (CamKII) and PGC1α axis to increase mitochondrial biogenesis and oxidative metabolism. These studies provide a fundamental framework for understanding the role of sarcoplasmic reticulum (SR)-Ca2+ cycling as an important factor in mitochondrial health and muscle metabolism. We propose that SLN can be targeted to enhance energy expenditure in muscle and prevent metabolic disease.
• Keywords: Ca(2+) signaling, CamKII, PGC1α, SERCA, Lipotoxicity, Mitochondrial biogenesis, Oxidative metabolism, Primary muscle myotubes, Sarcolipin, Skeletal muscle • Bioblast editor: Plangger M
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, nDNA;cell genetics
Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase
Coupling state: OXPHOS Pathway: N, NS HRR: Oxygraph-2k