Magnifico 2017 Oxid Med Cell Longev

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Publications in the MiPMap
Magnifico MC, Oberkersch RE, Mollo A, Giambelli L, Grooten Y, Sarti P, Calabrese GC, Arese M (2017) VLDL induced modulation of nitric oxide signalling and cell redox homeostasis in HUVEC. Oxid Med Cell Longev 2017:2697364.

» PMID: 29085553 Open Access

Magnifico MC, Oberkersch RE, Mollo A, Giambelli L, Grooten Y, Sarti P, Calabrese GC, Arese M (2017) Oxid Med Cell Longev

Abstract: High levels of circulating lipoprotein constitute a risk factor for cardiovascular diseases, and in this context, the specific role of the very-low-density lipoproteins (VLDL) is poorly understood. The response of human umbilical vein endothelial cells (HUVEC) to VLDL exposure was studied, especially focusing on the pathways involved in alteration of redox homeostasis and nitric oxide (NO) bioavailability. The results obtained by the analysis of the expression level of genes implicated in the NO metabolism and oxidative stress response indicated a strong activation of inducible NO synthase (iNOS) upon 24 h exposure to VLDL, particularly if these have been preventively oxidised. Simultaneously, both mRNA and protein expression of endothelial NO synthase (eNOS) were decreased and its phosphorylation pattern, at the key residues Tyr495 and Ser1177, strongly suggested the occurrence of the eNOS uncoupling. The results are consistent with the observed increased production of nitrites and nitrates (NOx), reactive oxygen species (ROS), 3-nitrotyrosine (3-NT), and, at mitochondrial level, a deficit in mitochondrial O2 consumption. Altogether, these data suggest that the VLDL, particularly if oxidised, when allowed to persist in contact with endothelial cells, strongly alter NO bioavailability, affecting redox homeostasis and mitochondrial function.


Bioblast editor: Kandolf G O2k-Network Lab: IT Rome Sarti P


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology  Pathology: Cardiovascular 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Permeabilized cells 


Coupling state: OXPHOS  Pathway: N, S, CIV  HRR: Oxygraph-2k 

Labels, 2017-12