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Lei 2021 Ecotoxicol Environ Saf

From Bioblast
Publications in the MiPMap
Lei C, Liao J, Li Q, Shi J, Zhang H, Guo J, Han Q, Hu L, Li Y, Pan J, Tang Z (2021) Copper induces mitochondria-mediated apoptosis via AMPK-mTOR pathway in hypothalamus of pigs. Ecotoxicol Environ Saf 220:112395.

Β» PMID: 34102394 Open Access

Lei Chaiqin, Liao Jianzhao, Li Quanwei, Shi Jian, Zhang Hui, Guo Jianying, Han Qingyue, Hu Lianmei, Li Ying, Pan Jiaqiang, Tang Zhaoxin (2021) Ecotoxicol Environ Saf

Abstract: Copper (Cu), one of the heavy metals, is far beyond the carrying capacity of the environment with Cu mining, industrial wastewater discharging and the use of Cu-containing pesticides. Intaking excess Cu can cause toxic effects on liver, kidney, heart, but few studies report Cu toxicity on brain tissue. It is noteworthy that most toxicity tests are based on rodent models, but large mammals chosen as animal models has no reported. To explore the relationship of the Cu toxicity and mitochondria-mediated apoptosis on hypothalamus in pigs, the content of Cu, histomorphology, mitochondrial related indicators, apoptosis, and AMPK-mTOR signaling pathway were detected. Results showed that Cu could accumulate in hypothalamus and lead to mitochondrial dysfunction, evidenced by the decrease of ATP production, activities of respiratory chain complex I-IV, and mitochondrial respiratory function in Cu-treated groups. Additionally, the genes and proteins expression of Bax, Caspase-3, Cytc in treatment group were higher than control group. Furthermore, the protein level of p-AMPK was enhanced significantly and p-mTOR was declined, which manifested that AMPK-mTOR signaling pathway was activated in Cu-treated groups. In conclusion, this study illuminated that the accumulation of Cu could cause mitochondrial dysfunction, induce mitochondria-mediated apoptosis and activate AMPK-mTOR pathway in hypothalamus. β€’ Keywords: AMPK-mTOR pathway, Apoptosis, Copper, Hypothalamus, Mitochondrion β€’ Bioblast editor: Reiswig R β€’ O2k-Network Lab: SG Singapore Hausenloy DJ

Labels: MiParea: Respiration, Pharmacology;toxicology 

Organism: Pig  Tissue;cell: Nervous system 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, ROX  HRR: Oxygraph-2k