Lara-Marqueza 2020 J Funct Foods
| Lara-MΓ‘rquez M, BΓ‘ez-MagaΓ±a M, Raymundo-Ramos C, Spagnuolo PA, MacΓas-RodrΓguez L, Salgado-Garciglia R, Ochoa-Zarzosa A, LΓ³pez-Meza JE (2020) Lipid-rich extract from Mexican avocado (Persea americana var. drymifolia) induces apoptosis and modulates the inflammatory response in Caco-2 human colon cancer cells. J Funct Foods 64:103658. |
Β» Open Access
Lara-Marquez M, Baez-Magana M, Raymundo-Ramos C, Spagnuolo PA, Macias-Rodriguez L, Salgado-Garciglia R, Ochoa-Zarzosa A, Lopez-Meza JE (2020) J Funct Foods
Abstract: Colorectal cancer (CRC) is the third most common type of cancer worldwide. Chemotherapy used in CRC patients has severe side effects. As an alternative, the search to identify natural compounds increases the opportunity to select therapeutics. In this work, the cytotoxic effect of a lipid extract (LEAS) from Mexican native avocado seed (Persea americana var. drymifolia) on colon cancer cells Caco-2 was determined. LEAS was cytotoxic towards Caco-2 cells (IC50β―=β―28β―Β΅g/ml), inducing apoptosis through the activation of caspases 8 and 9. Also, LEAS induced loss of membrane mitochondrial potential, inhibited fatty acid oxidation and increased the superoxide production and mitochondrial ROS. Furthermore, LEAS stimulated secretion of cytokines IL-6 (~500%), IL-8 (~400%) and IL-10 (~150%); whereas IL-1Ξ² secretion was inhibited (~50%). The results suggest that LEAS induces apoptosis on Caco-2 cells, indicating that avocado is a source of functional food products that can reduce the risk for development of cancer. β’ Keywords: Colon cancer, Long-chain lipids, Apoptosis, Avocado, Persea americana β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology
Pathology: Cancer
Stress:Cell death
Organism: Human
Tissue;cell: Endothelial;epithelial;mesothelial cell
Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET
HRR: Oxygraph-2k
Labels, 2020-02
