Krischek 2019 Meat Sci
|Krischek C, Popp J, Sharifi AR (2019) Biochemical alterations in the Musculus triceps brachii and Musculus longissimus thoracis during early postmortem period in pigs. Meat Sci 152:121-26.|
Abstract: Muscle-to-meat-transition is influenced by alterations of the energy metabolism. Porcine Musculus triceps brachii (MT) consisted of more fast-twitch-glycolytic muscle fibers and samples, collected 0, 10 and 20 min after slaughter (p.m.), showed higher mitochondrial respiratory activities and ATP concentrations than Musculus longissimus thoracis (LT) samples. Enzyme activities in MT were higher at 0 min (glycogen phosphorylase (GP)), 10 min (GP, citrate synthase (CS)) and at 20 min p.m. (CS). However, LT results were higher at 0 min (lactate dehydrogenase (LDH)), 10 min (phosphofructokinase (PFK), LDH) and at 20 min p.m. (PFK, F0F1-ATPase (F0F1)). Between 0 min and 10 min p.m. CS activities decreased in LT and MT samples, PFK increased in LT and GP in MT samples. Between 10 min and 20 min p.m. PFK and LDH decreased in LT and GP in MT samples, whereas F0F1 increased in LT and CS in MT samples. The data indicate that muscles with different mitochondria contents show clearly different energy metabolism characteristics.
• Keywords: Metabolic enzymes, Mitochondrial respiration, Musculus longissimus thoracis, Musculus triceps brachii, Pig, Postmortem metabolism • Bioblast editor: Plangger M • O2k-Network Lab: DE Goettingen Krischek C, DE Goettingen Moerlein D
Labels: MiParea: Respiration
Organism: Pig Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue Enzyme: Complex V;ATP synthase Regulation: ADP, ATP, AMP Coupling state: OXPHOS Pathway: N HRR: Oxygraph-2k