Kimball 2016 Thesis

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Kimball RL (2016) The role of hypoxia on pyruvate kinase M2, mammalian target of rapamycin, mitochondrial function, and cell invasion in the trophoblast. Master Thesis p55.

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Kimball RL (2016) Thesis Brigham Young University

Abstract: Intrauterine growth restriction (IUGR) is a disease that affects many pregnant women and their growing fetuses. Hypoxia and shallow invasion are key characteristics of IUGR. A lack of oxygen has been shown to affect the mitochondria, which produces the energy in the cell. Pyruvate Kinase M2 (PKM2) is a metabolic enzyme known to be involved during hypoxia. Our objective was to determine the role of hypoxia in mitochondrial function, trophoblast cell invasion, and PKM2.

Human placenta tissues were collected at term to characterize the mitochondria in the cells. Human trophoblast cells were used to determine PKM2 expression. Treatment was with hypoxia, Shikonin, or Rapamycin to observe mitochondrial function and invasion characteristics of the trophoblast cells. The cells were lysed after treatment and western blots were performed to view PKM2 expression.

Hypoxic treated cells showed a decrease in mitochondrial function, invasion, and nuclear and cytostolic PKM2. Shikonin treated cells showed a decrease in invasion and nuclear PKM2, and an increase in mitochondrial function.

Keywords: Human cytotrophoblast Sw71 cells

O2k-Network Lab: US UT Provo Bikman BT


Labels: MiParea: Respiration  Pathology: Other  Stress:Oxidative stress;RONS  Organism: Human  Tissue;cell: Genital, Other cell lines  Preparation: Permeabilized cells 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 

2016-06