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Jang 2017 Biol Open

From Bioblast
Publications in the MiPMap
Jang DH, Seeger SC, Grady ME, Shofer FC, Eckmann DM (2017) Mitochondrial dynamics and respiration within cells with increased open pore cytoskeletal meshes. Biol Open 6:1831-9.

Β» PMID: 29109116

Jang DH, Seeger SC, Grady ME, Shofer FC, Eckmann DM (2017) Biol Open

Abstract: The cytoskeletal architecture directly affects the morphology, motility, and tensional homeostasis of the cell. In addition, the cytoskeleton is important for mitosis, intracellular traffic, organelle motility, and even cellular respiration. The organelle responsible for a majority of the energy conversion for the cell, the mitochondrion, has a dependence on the cytoskeleton for mobility and function. In previous studies, we established that cytoskeletal inhibitors altered the movement of the mitochondria, their morphology, and their respiration in human dermal fibroblasts. Here, we use this protocol to investigate applicability of power law diffusion to describe mitochondrial locomotion, assessment of rates of fission and fusion in healthy and diseased cells, and differences in mitochondria locomotion in more open networks either in response to cytoskeletal destabilizers or by cell line. We found that mitochondria within fibrosarcoma cells and within fibroblast cells treated with an actin-destabilizing toxin resulted in increased net travel, increased average velocity, and increased diffusion of mitochondria when compared to control fibroblasts. Although the mitochondria within the fibrosarcoma travel further than mitochondria within their healthy counterparts, fibroblasts, the dependence on mitochondria for respiration is much lower with higher rates of hydrogen peroxide production and was confirmed using the Oroboros O2k. We also found that rates of fission and fusion of the mitochondria equilibrate despite significant alteration of the cytoskeleton. Rates ranged from 15% to 25%, where the highest rates were observed within the fibrosarcoma cell line. This result is interesting because the fibrosarcoma cell line does not have increased respiration metrics including when compared to fibroblast. Mitochondria travel further, faster, and have an increase in percent mitochondria splitting or joining while not dependent on the mitochondria for a majority of its energy production. This study illustrates the complex interaction between mitochondrial movement and respiration through the disruption of the cytoskeleton.

Β© 2017. Published by The Company of Biologists Ltd. β€’ Keywords: Cytoskeletal, Dynamics, Mitochondria, Respiration, Amplex Red β€’ Bioblast editor: Kandolf G β€’ O2k-Network Lab: US PA Philadelphia Jang DH

Labels: MiParea: Respiration, mt-Structure;fission;fusion, mt-Medicine  Pathology: Cancer  Stress:Oxidative stress;RONS  Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell, Fibroblast  Preparation: Intact cells, Permeabilized cells 

Coupling state: LEAK, ROUTINE, ET  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

2017-11, AmR