MiPNet21.11 IOC116 Innsbruck AT
 Innsbruck AT, 2016 Nov 21-25. Oroboros O2k-Workshop on O2k-Fluorometry - advanced, IOC116. |
Oroboros (2016-11-21) Mitochondr Physiol Network
Abstract:
Oroboros O2k-Workshop on O2k-Fluorometry - advanced. Innsbruck, Austria; 2016 Nov 21-25.
• O2k-Network Lab: AT_Innsbruck_Oroboros
Feedback
- It was my pleasure to participate, always useful and great fun. - Spinazzi Marco
Programme
Monday Nov 21
| Time | Topic | Materials | |
|---|---|---|---|
| Arrival | Hotel Grauer Bär | ||
| 18:00 | Dinner | ||
| 19:30 | Get-together and introduction of participants and their research interests - a welcome by Oroboros Instruments. | O2k-Network |
Tuesday Nov 22
| Time | Topic | Materials | |
|---|---|---|---|
| 08:30 |  |
Erich Gnaiger
O2k-Fluorometry: Respiration, mt-membrane potential and H2O2 production. |
O2k-Fluorometer |
| 09:30 | 
|
Carolina Doerrier, Erich Gnaiger
Respiration and H2O2 production in permeabilized HEK cells - O2k-Fluo demo experiment. |
MiPNet21.06 SUIT RP, Makrecka-Kuka 2015 Biomolecules |
| 10:30 | Coffe/tea break | ||
| 10:45 | 
|
Carolina Doerrier
Respiration and H2O2 production in permeabilized HEK cells - Hands-on experiment with eight O2k-Fluorometers, 16 O2k-Chambers. |
Hydrogen peroxide |
| 12:30 | Lunch | ||
| 14:00 | 
|
DatLab analysis and collection of experimental data. | MitoPedia: DatLab |
| 15:30 | 
|
Anthony Moore
IOC lecture: Investigations into the multi-faceted roles of the alternative oxidases: are they a reflection of the nature of the hydrophobic cavity? |
Moore 2016 Abstract IOC116 |
| 16:00 | Coffee/tea break | ||
| 16:15 | 
|
Oroboros-Team and Guest Tutors
Data discussion, SUIT protocol interpretation, summary. |
MitoPedia: SUIT |
| 18:00 | Dinner | ||
| O2k- and MiP-perspectives | |||
| 19:30 | 
|
Sobotka O, Kucera O, Stankova P, Endlicher R, Nozickova K, Banni A, Cervinkova Z
Mitochondrial respiration in fatty liver. |
Sobotka 2016 Abstract IOC116 |
| 19:45 | 
|
Nielsen B, Woertwein G, Cejvanovic V, Hansen AR, Weimann A, Bjerring PN, Poulsen HE, Joergensen MB
The function of mitochondria and oxidative stress after antidepressants treatment in rats. |
Nielsen 2016 Abstract IOC116 |
| 20:00 | 
|
Morato L, Guillot de Suduiraut MI, Grosse J, Zanoletti O, Riccio O, Fournier C, Sandi C
Impaired mitochondrial function mediates early life stress-induced depression. |
Morato 2016 Abstract IOC116 |
| 20:15 | 
|
Wyss RK, Mendez-Carmona N, Nieves Sanz M, Tevaearai Stahel HT, Longnus SL
|
Wyss 2016 Abstract IOC116 |
Wednesday Nov 23
| Time | Topic | Materials | |
|---|---|---|---|
| 08:30 | 
|
Zuzana Sumbalova
O2k-potentiometric measurement of mt-membrane potential with TPP+: TPP+ calibration and demo with mouse brain isolated mitochondria. |
O2k-TPP+ ISE-Module |
| 10:30 | Coffe/tea break | ||
| 10:45 | 
|
Demo with mouse brain isolated mitochondria. | O2k-TPP+ ISE-Module |
| 12:30 | Lunch | ||
| 14:00 |
|
TPP+ or O2k-Fluorometry: Safranin or TMRM - hands-on. | Krumschnabel 2014 Methods Enzymol |
| 16:00 | Coffee/tea break | ||
| 16:15 |
|
Zuzana Sumbalova
Data analysis and discussion. |
Krumschnabel 2015 Methods Mol Biol |
| 18:00 | Dinner | ||
| O2k- and MiP-perspectives | |||
| 19:30 | 
|
Komlodi T, Geibl F, Sassani M, Adam-Vizi V, Tretter L
The effect of ΔΨmt and ΔpH on mitochondrial reactive oxygen species production - A comparative study using mitochondria isolated from different mammalian tissues. |
Komlodi 2016 Abstract IOC116 |
| 19:45 | 
|
Cecatto C, Silva JC, Wajner A, Godoy KS, Ribeiro, RT, Amaral AU, Wajner M
cis-4-Decenoic and decanoic acids impair mitochondrial energy, redox and Ca2+ homeostasis and induce mitochondrial permeability transition pore opening in rat brain and liver. |
Cecatto 2016 Abstract IOC116 |
| 20:00 | 
|
Krizova J, Macakova M, Bohuslavova B, Klima J, Ellederova Z, Zeman J, Motlik J, Hansikova H
Complex I-dependent respiration decline is a marker of Huntington`s disease in spermatozoa of transgenic minipig model. |
Krizova 2016 Abstract IOC116 |
Thursday Nov 24
| Time | Topic | Materials | |
|---|---|---|---|
| 08:30 | 
|
Christos Chinopoulos
Determination of ADP-ATP exchange rate in isolated mitochondria. |
Magnesium Green |
| 10:30 | Coffe/tea break | ||
| 10:45 | 
|
Christos Chinopoulos
O2k-Fluo measurement (Mg-Green) of the ~P/O2 ratio in isolated mitochondria - demo experiment. |
O2k-Fluorometer |
| 12:30 | Lunch | ||
| 14:00 |
|
Christos Chinopoulos
Data analysis and summary of experimental results. |
MiPNet17.05 O2k-Fluo LED2-Module |
| 16:00 | Coffee/tea break | ||
| 16:30 | 
|
Glühwein at the Christkindl market in Innsbruck Old Town. | Glühwein manual |
| 18:00 | Dinner | ||
|
Departure, Friday Nov 25 |
Abstracts
| Has title | |
|---|---|
| Komlodi 2016 Abstract IOC116 | Komlodi T, Geibl F, Sassani M, Adam-Vizi V, Tretter L (2016) The effect of ΔΨmt and ΔpH on mitochondrial reactive oxygen species production - A comparative study using mitochondria isolated from different mammalian tissues. Mitochondr Physiol Network 21.11 |
| Cecatto 2016 Abstract IOC116 | Cecatto C, Silva JC, Wajner A, Godoy KS, Ribeiro, RT, Amaral AU, Wajner M (2016) cis-4-Decenoic and decanoic acids impair mitochondrial energy, redox and Ca2+ homeostasis and induce mitochondrial permeability transition pore opening in rat brain and liver. Mitochondr Physiol Network 21.11 |
| Poddar 2016 Abstract IOC116 | Poddar J, Banerjee A, Mukherjee A, Chakrabarti S (2016) Effects of glucose hypometabolism on mitochondrial functions in rat brain and in SHSY5Y cells in culture. Mitochondr Physiol Network 21.11 |
| Sobotka 2016 Abstract IOC116 | Sobotka O, Kucera Otto, Stankova P, Endlicher R, Nozickova K, Banni A, Cervinkova Z (2016) Mitochondrial respiration in fatty liver. Mitochondr Physiol Network 21.11 |
| Nielsen 2016 Abstract IOC116 | Nielsen B, Woertwein G, Cejvanovic V, Hansen AR, Weimann A, Bjerring PN, Poulsen HE, Joergensen MB (2016) The function of mitochondria and oxidative stress after antidepressants treatment in rats. Mitochondr Physiol Network 21.11 |
| Krizova 2016 Abstract IOC116 | Krizova J, Macakova M, Bohuslavova B, Klima J, Ellederova Z, Zeman J, Motlik J, Hansikova H (2016) Complex I-dependent respiration decline is a marker of Huntington`s disease in spermatozoa of transgenic minipig model. Mitochondr Physiol Network 21.11 |
| Morato 2016 Abstract IOC116 | Morato L, Guillot de Suduiraut MI, Grosse J, Zanoletti O, Riccio O, Fournier C, Sandi C (2016) Impaired mitochondrial function mediates early life stress-induced depression. Mitochondr Physiol Network 21.11 |
| Wyss 2016 Abstract IOC116 | Wyss RK, Mendez-Carmona N, Nieves Sanz M, Tevaearai Stahel HT, Longnus SL (2016) Tolerance of cardiac mitochondria to ischemia and reperfusion. Mitochondr Physiol Network 21.11 |
| Moore 2016 Abstract IOC116 | Moore AL (2016) Investigations into the multi-faceted roles of the alternative oxidases: are they a reflection of the nature of the hydrophobic cavity? Mitochondr Physiol Network 21.11 |
| ... further results | |
Team and guest tutors
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
Doerrier Carolina, Chief Scientific Officer (CSO), Mitochondrial Physiology Specialist, Oroboros Instruments
Sumbalova Zuzana, Principal Investigator (PI), Mitochondrial Physiology Specialist, Oroboros Instruments
Chinopoulos Christos, Department of Medical Biochemistry, Semmelweis University, Budapest (HU)
Meszaros Andras, University of Szeged (HU)
Komlodi Timea: Department of Medical Biochemistry, Semmelweis University, Budapest (HU)
Sobotka Ondrej: Department of Physiology, Charles University in Prague, Hradec Kralove (CZ)
Participants
| Participant | Institution | |
|---|---|---|
| Aparicio Trejo Omar Emiliano | MX Mexico City Pedraza Chaverri J: Department of Biology, University of Mexico, Mexico City (MX) | |
| Bhuvanachandran Nair Syam | SE Gothenburg Hagberg H: Institute of Neuroscience and Physiology, University of Gothenburg (SE) | |
|
Casado Pinna Marta | ES Valencia Casado Pinna M: Department of Molecular and Cellular Pathology and Therapy, Instituto de Biomedicina de Valencia (ES) |
|
|
Cecatto Cristiane | BR Porto Alegre Souza DOG: Universidade Federal do Rio Grande do SuL, Porto Alegre(BR) |
| Einer Claudia | DE Munich Zischka H: Helmholtz Centre Munich (DE) | |
| Grewal Rekha | DE Giessen Eckert GP: Department of Nutrition in Prevention & Therapy, Justus Liebig University, Giessen (DE) | |
| Goy Christine | DE Duesseldorf Haendeler J: Leibniz Research Institute for Environmental Medicine, Molecular Aging Research, Duesseldorf (DE) | |
| Hey-Mogensen Martin | US WA Seattle Hey-Mogensen M: Novo Nordisk Research Center, Seattle (US) | |
|
|
Krizova Jana | CZ Prague Zeman J: Charles University and General University Hospital in Prague (CZ) |
|
|
Komlodi Timea | HU Budapest Tretter L: Department of Medical Biochemistry, Semmelweis University, Budapest (HU)  COST Action CA15203 MitoEAGLE: Short-term scientific mission |
| Kraunsoee Regitze | DK Copenhagen Dela F: Department of Biomedical Sciences, University of Copenhagen (DK) | |
| Matsumoto Junichi | JP Sapporo Yokota T: Department of Cardiovascular Medicine, Hokkaido University, Sapporo (JP) | |
|
Meszaros Andras | HU Szeged Boros M: University of Szeged (HU) COST Action CA15203 MitoEAGLE: Short-term scientific mission |
|
|
Moore Anthony L | UK Brighton Moore AL: University of Sussex, Brighton (UK) |
|
|
Morato Fornaguera Laia | CH Lausanne Sandi C: EPFL, Lausanne (CH) |
|
Neves Pedro | AT Innsbruck OROBOROS: Oroboros Instruments(AT) |
| Nielsen Birgitte | DK Copenhagen Poulsen HE: Rigshospitalet Copenhagen, Lab of clinical Pharamacology (DK) | |
| Schaefer Carmina | DE Giessen Eckert GP: Department of Nutrition in Prevention & Therapy, Justus Liebig University, Giessen (DE) | |
| Sigl Reinhard | AT Innsbruck OROBOROS: Oroboros Instruments (AT) | |
|
|
Sobotka Ondrej | CZ Hradec Kralove Cervinkova Z: Department of Physiology, Charles University in Prague, Hradec Kralove (CZ) |
|
Spinazzi Marco | BE Leuven Spinazzi M: KU Leuven Center for Human, VIB Center for the Biology of Disease (BE) |
| Weir Mary | CA Antigonish Kane DA: St. Francis Xavier University, Antigonish (CA) | |
|
|
Wyss Rahel | CH Bern Longnus SL: Bern University Hospital, Department of Cardiovascular Surgery (CH) |
Demo DatLab file for mitochondrial membrane potential
General information
- This O2k-Workshop for advanced users provides a short introduction to HRR. The O2k-Fluo LED2-Module and O2k-Fluorometry will be presented in overview lectures and hands-on sessions; protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin, TMRM), ATP production (Mg green), and Ca2+ (Ca green) will be discussed.
- » Information on travel and venue: IOC Innsbruck.
- Venue
- Hotel Grauer Bär
- Universitätsstrasse 5-7, 6020 Innsbruck
- Tel: +43 (0) 512 5924
- Our prices include accommodation at Hotel Grauer Bär, full board, coffee breaks, spa area
- Venue
- Registration
- Please fill in the registration form and send it to [email protected]
- » Registration pdf
- Registration
- Support
- IOC student scholarships are available for this IOC. More information? See here.
- Support
- O2k perspectives - abstracts
- Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.
- » Abstract guidelines
- O2k perspectives - abstracts
- IOC recommended reading
- Contact
- [email protected]
- Oroboros Instruments, high-resolution respirometry
- Schöpfstrasse 18, A-6020 Innsbruck, Austria
- Tel: +43 512 566796; Fax: +43 512 566796 20
- Contact
- Mitochondria and cell research
- Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
- Project support
O2k-Workshops are listed as MitoGlobal Events.
Labels:
ORO, IOC, 2016, MitoFit
