Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Homsani 2013 Int J High Dilution Res

From Bioblast
Publications in the MiPMap
Homsani F, Barbosa GM, Fernandes L, Monteiro Siqueira C, dos Santos LH, el-Bach T, Souza Santos AL, Quaresma CH (2013) Evaluation of Alterations in adhesion rates of Candida albicans and in respiration cellular induced by Candida albicans type RC nosodes. Int J High Dilution Res 12:152-3.

Β» PDF

Homsani F, Barbosa GM, Fernandes L, Monteiro Siqueira C, dos Santos LH, el-Bach T, Souza Santos AL, Quaresma CH (2013) Int J High Dilution Res

Abstract: Candidiasis is an opportunist infection, caused by yeast of the genus Candida. It usually affects immunossupressed and critical patients, and may progress to a systemic infection with high morbidity and mortality rates. Candida albicans is its most common causing agent. The yeast adhesion to the host epithelial cells is a great stimulator for the hyphae formation, which is the invasive form of this fungal[1]. Nosodes are homeopathic medicines employed with great success in the clinical treatment of these infections and it is prepared from organic products chemically undefined[2]. Living nosodes RC are prepared from the etiologic agent in integrity state and current studies demonstrate their therapeutic potential[3,4].

To evaluate possible alterations induced by RC-type C. albicans nosodes in cellular respiration and in the adhesion rates of Candida albicans to MA104 cells.

RC-type Candida albicans nosodes were prepared according to the homeopathic pharmacopeia; one part of C. albicans suspension (108 cell/ml) was diluted in 9 parts of sterile distilled water and submitted to 100 mechanical successions. This sample was subjected to mechanical sucussions 100, leading to 1x. This procedure was repeated successively until 12x and 30x. C. albicans adhesion rate quantification was performed by flow cytometry after treating MA104 cells for 1, 18 and 40 days with 10% of Candida RC 12x and 30x. Variations in the respiration parameters of Ma104 cells treated with nosodes for 2 and 4 days were evaluated by high-resolution respirometry[5]. Several experimental conditions were compared to their respective control (cells treated with dinamized and not dinamized sterile distilled water).

A decrease in adherence rates, around 20%, was detected when MA104 cells were treated for 18 days with a tendency to decrease fluorescence intensity in all treatments. In addition, treatment with nosodes decreased the MA104 cells respiratory capacity.

Results indicate that nosodes modify both the yeasts adhesion rate and maximum respiratory capacity; it is still not possible to say whether there is a direct correlation between these cellular alterations. β€’ Keywords: Candida albicans, Systemic candidiasis, Nosodes, Adhesion rates, Cellular respiration


Labels: MiParea: Respiration 


Organism: Fungi 




HRR: Oxygraph-2k