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Hoefler 2016 Physiol Plant

From Bioblast
Publications in the MiPMap
Höfler S, Lorenz C, Busch T, Brinkkötter M, Tohge T, Fernie AR, Braun HP, Hildebrandt TM (2016) Dealing with the sulfur part of cysteine: four enzymatic steps degrade l-cysteine to pyruvate and thiosulfate in Arabidopsis mitochondria. Physiol Plant 157:352-66..

» PMID: 27105581

Hoefler S, Lorenz C, Busch T, Brinkkoetter M, Tohge T, Fernie AR, Braun HP, Hildebrandt TM (2016) Physiol Plant

Abstract: Amino acid catabolism is essential for adjusting pool sizes of free amino acids and takes part in energy production as well as nutrient remobilization. The carbon skeletons are generally converted to precursors or intermediates of the tricarboxylic acid cycle. In the case of cysteine, the reduced sulfur derived from the thiol group also has to be oxidized in order to prevent accumulation to toxic concentrations. Here we present a mitochondrial sulfur catabolic pathway catalyzing the complete oxidation of l-cysteine to pyruvate and thiosulfate. After transamination to 3-mercaptopyruvate, the sulfhydryl group from l-cysteine is transferred to glutathione by sulfurtransferase 1 and oxidized to sulfite by the sulfur dioxygenase ETHE1. Sulfite is then converted to thiosulfate by addition of a second persulfide group by sulfurtransferase 1. This pathway is most relevant during early embryo development and for vegetative growth under light-limiting conditions. Characterization of a double mutant produced from Arabidopsis thaliana T-DNA insertion lines for ETHE1 and sulfurtransferase 1 revealed that an intermediate of the ETHE1 dependent pathway, most likely a persulfide, interferes with amino acid catabolism and induces early senescence.

© 2016 Scandinavian Plant Physiology Society.

O2k-Network Lab: DE Duesseldorf Grieshaber MK, DE Hannover Hildebrandt T

Labels: MiParea: Respiration 

Organism: Plants 

Preparation: Oxidase;biochemical oxidation, Intact cells 

Pathway: CIV, Other combinations  HRR: Oxygraph-2k 

2016-06, MitoFit 2021 Photosynthesis 

  • Huete-Ortega et al (2021) Substrate-uncoupler-inhibitor-titration protocols for photosynthesis in Chlamydomonas reinhardtii. MitoFit Preprints 2021 (in prep).