Havird 2018 bioRxiv
|Havird JC, Noe GR, Link L, Torres A, Logan DC, Sloan DB, Chicco AJ (2018) Assessing mitochondrial function in angiosperms with highly divergent mitochondrial genomes. bioRxiv doi: https://doi.org/10.1101/448902.|
Abstract: Angiosperm mitochondrial (mt) genes are generally slow-evolving, but multiple lineages have undergone dramatic accelerations in rates of nucleotide substitution and extreme changes in mt genome structure. While molecular evolution in these lineages has been investigated, very little is known about their mt function. Here, we develop a new protocol to characterize respiration in isolated plant mitochondria and apply it to species of Silene with mt genomes that are rapidly evolving, highly fragmented, and exceptionally large (~11 Mbp). This protocol, complemented with traditional measures of plant fitness, cytochrome c oxidase activity assays, and fluorescence microscopy, was used to characterize inter- and intraspecific variation in mt function. Contributions of the individual "classic" OXPHOS complexes, the alternative oxidase, and external NADH dehydrogenases to overall mt respiratory flux were found to be similar to previously studied angiosperms with more typical mt genomes. Some differences in mt function could be explained by inter- and intraspecific variation, possibly due to local adaptation or environmental effects. Although this study suggests that these Silene species with peculiar mt genomes still show relatively normal mt function, future experiments utilizing the protocol developed here can explore such questions in a more detailed and comparative framework.
• Keywords: Alternative NADH dehydrogenase, Cytonuclear interactions, Flux control factor, Mitochondrial respiration, Oroboros Oxygraph 2K, SUIT protocol • Bioblast editor: Plangger M • O2k-Network Lab: BR Rio de Janeiro Galina A, US CO Fort Collins Chicco AJ
Labels: MiParea: Respiration, Instruments;methods, mtDNA;mt-genetics
Preparation: Isolated mitochondria Enzyme: Complex IV;cytochrome c oxidase Regulation: Flux control, Inhibitor Coupling state: LEAK, OXPHOS Pathway: N, S, CIV, NS HRR: Oxygraph-2k