Hatefi 1961 Biochim Biophys Acta

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Hatefi Y, Haavik AG, Jurtshuk P (1961) Studies on the electron transport system XXXI. DPNH-cytochrome c reductase II. Biochim Biophys Acta 52:106-18.

» PMID: 13905330

Hatefi Y, Haavik AG, Jurtshuk P (1961) Biochim Biophys Acta

Abstract: A highly active DPNH-cytochrome c reductase has been isolated from beef-heart mitochondria. The best preparations of the enzyme catalyze the reduction by DPNH of approx. 50–60 μmoles cytochrome c/min/mg protein at 38°. The enzymic activity is completely inhibited by Amytal, p-chloromercuriphenyl sulfonate, antimycin A, SN-5949 or 2-nonyl-4-hydroxyquinoline-N-oxide, and is stimulated by EDTA. The preparation contains DPNH flavoprotein, cytochromes b and c1, Coenzyme Q and non-heme iron and is essentially free of succinic-cytochrome c reductase as well as cytochrome oxidase activity.

Keywords: DPNH-cytochrome c reductase, Beef heart mitochondria


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Organism: Bovines  Tissue;cell: Heart  Preparation: Isolated mitochondria  Enzyme: Complex IV;cytochrome c oxidase  Regulation: Substrate  Coupling state: ET 


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