Harper 2012 Abstract Bioblast
|Harper ME, Yin H, Pasut A, Soleimani VD, Bentzinger CF, Antoun G, Thorn S, Seale P, Fernando P, Van IJcken W Frank Grosveld F, Dekemp RA, Boushel RC, Rudnicki MA (2012) Metabolic effects of microRNA-133 and its antagomir in mice. Mitochondr Physiol Network 17.12.|
Event: Bioblast 2012
The unequivocal identification of functional brown adipose tissue (BAT) in adult humans (reviewed in ) has led to a resurgence of interest in this unique tissue and its potential in novel anti-obesity therapies [2,3]. Unlike white adipose tissue, BAT is rich with mitochondria. BAT is capable of remarkably high rates of uncoupled respiration due to the activity of uncoupling protein-1 (UCP-1). The cellular origins of brown adipocytes are not well understood, but the transcription factor Prdm16 is necessary and sufficient in establishing brown adipocyte lineage . In vitro loss-of-function of Prdm16 induces myogenic differentiation of committed preadipocytes from BAT, while gain-of-function induces committed myoblasts to differentiate into brown adipocytes . Satellite cells are adult skeletal muscle stem cells, located beneath the basal lamina of muscle, and when activated, they proliferate and differentiate into multi-nucleated muscle cells. In this study we show that brown adipocyte determination of satellite cells is controlled by miR-133. Loss-of-function of miR-133 during muscle regeneration in mice commits satellite cells to the brown adipocyte lineage, causing local uncoupled respiration, increased glucose uptake and whole body thermogenesis, as well as decreased diet–induced obesity.
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• Keywords: Uncoupling protein-1 (UCP1), Brown fat, Thermogenesis, Obesity
Organism: Mouse Tissue;cell: Skeletal muscle, Fat Preparation: Intact organism, Permeabilized cells
Coupling state: LEAK
Mary-Ellen Harper (1), Hang Yin, Alessandra Pasut, Vahab D Soleimani, C Florian Bentzinger, Ghadi Antoun, Stephanie Thorn, Patrick Seale, Pasan Fernando, Wilfred van IJcken, Frank Grosveld, Robert A Dekemp, Robert Boushel, Michael A Rudnicki
(1) Mitochondrial Bioenergetics Laboratory, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada; Email: [email protected]
4. Seale P, Bjork B, Yang W, Kajimura S, Chin S, Kuang S, Scimè A, Devarakonda S, Conroe HM, Erdjument-Bromage H, Tempst P, Rudnicki MA, Beier DR, Spiegelman BM (2008) PRDM16 controls a brown fat/skeletal muscle switch. Nature 454: 961-796. Open Access