Goncalves 2011 Abstract IOC65
|Gonçalves RL, Barretoc RFSM, Polycarpod CR, Castro SL, Gadelhae FR, Oliveira MF (2011) Comparative assessment of mitochondrial function on epimastigotes and bloodstream trypomastigotes of Trypanosoma cruzi. MiPNet16.03.|
Trypanosoma cruzi is a hemoflagellate protozoan that causes Chagas’ disease. T. cruzi life-cycle is complex involving different evolutive forms that experience striking differences in their environmental condition. Here we carried out a functional assessment of mitochondrial function in two distinct T. cruzi forms: the insect stage, epimastigote and the freshly isolated bloodstream trypomastigote. We observed that in comparison to epimastigotes, bloodstream trypomastigotes facilitate electrons entry into the electron transport chain increasing Complex II-III activity. Curiously, cytochrome c oxidase (CIV) activity and the expression of CIV subunit IV were reduced in bloodstream forms, creating an “electron bottleneck” that favored increased electron leak and H2O2 formation. We propose that the oxidative preconditioning provided by this mechanism would confer a protection to the bloodstream trypomastigotes against host immune response. Thus, mitochondrial remodeling during the T. cruzi life-cycle can represent a key metabolic adaptation for parasite survival in different environments.
• Keywords: Energy metabolism, Reactive oxygen species, Trypanosomatids
• O2k-Network Lab: BR Rio de Janeiro Oliveira MF
Labels: MiParea: Respiration, Developmental biology
Enzyme: Complex IV;cytochrome c oxidase
a.Laboratório de Bioquímica de Resposta ao Estresse UFRJ c. Laboratório de Biologia Celular, Instituto Oswaldo Cruz d. Laboratório de Biologia Molecular, Programa de Biologia Molecular e Biotecnologia, Instituto de Bioquímica Médica e. Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.