Gnaiger 2000 Transpl Int

Gnaiger E, Kuznetsov AV, Rieger G, Amberger A, Fuchs A, Stadlmann S, Eberl T, Margreiter R (2000) Mitochondrial defects by intracellular calcium overload versus endothelial cold ischemia/reperfusion injury. Transpl Int 13:S555-7.

» - PMID: 11112072

Gnaiger Erich, Kuznetsov AV, Rieger G, Amberger A, Fuchs A, Stadlmann S, Eberl T, Margreiter R (2000) Transpl Int

Abstract: Questions as to the critical stress factor and primary targets of cold ischemia/reperfusion (CIR) injury were addressed by comparing mitochondrial defects caused by (1) CIR injury and (2) intracellular Ca²⁺ overload. CIR was simulated in transformed human umbilical vein endothelial cell cultures (tEC) by 8 h cold anoxia in University of Wisconsin solution and reoxygenation at 37 °C. Intracellular Ca²⁺ concentrations were changed by permeabilization of suspended cells with digitonin in culture medium (RPMI, 0.4 mM Ca²⁺). Binding of free Ca²⁺ by ethylene glycol-bis(β-aminoethylether)- N,N,N',N'-tetraacetic acid in RPMI or mitochondrial incubation medium served as controls. Extracellular Ca²⁺ protected the cell membrane against permeabilization. Mitochondrial functions were determined before and after permeabilization of the cell membrane. After CIR, mitochondrial respiratory capacity declined, but oxygen consumption remained coupled to adenosine triphosphate (ATP) production. In contrast, Ca²⁺ overload caused uncoupling of mitochondrial respiration. High intracellular Ca²⁺ overload, therefore, does not reproduce cold ischemia/ reperfusion injury in endothelial cells. • Keywords: Ischemia/reperfusion injury, Endothelial cells, Plasma membrane, Intracellular Ca²⁺, Mitochondrial respiratory chain, Oxidative phosphorylation

• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Oroboros

Cited by

3 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/11112072/

Labels: MiParea: Respiration

Stress:Ischemia-reperfusion, Oxidative stress;RONS Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell, HUVEC Preparation: Intact cells, Permeabilized cells, Oxidase;biochemical oxidation

Regulation: Calcium Coupling state: ROUTINE, OXPHOS

HRR: Oxygraph-2k, MitoFit 2021 PLT"MitoFit 2021 PLT" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.