Giovarelli M

From Bioblast
Jump to: navigation, search


e-COST MitoEAGLE
e-COST MitoEAGLE countries
MitoGlobal
MitoEAGLE
News and Events         About COST Action MitoEAGLE         Working Groups         MiP/MitoEAGLE Conference, WG and MC Meeting 2019 Belgrade RS         MitoEAGLE Preprint States and rates         Short-Term Scientific Missions         Inclusiveness Target Countries         Management Committee         Members    


MiPsociety
COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping


 

Giovarelli M


MitoPedia topics: EAGLE 

COST: Member

COST WG2: WG2


COST ECI: ECI


Name Giovarelli Matteo, PhD

ORCID ID

Institution Unit of Clinical Pharmacology,

Department of Biomedical and Clinical Sciences,

"Luigi Sacco" University Hospital,

University of Milano, IT

Address Via GB Grassi 74, 20157
City Milan
State/Province
Country Italy
Email mgiovarelli@gmail.com
Weblink
O2k-Network Lab IT Milan Clementi E


Labels:



Publications

 PublishedReference
Gnaiger 2019 MitoFit Preprint Arch2019Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6.
Pedrotti 2019 Sci Adv2019Pedrotti S, Caccia R, Neguembor MV, Garcia-Manteiga JM, Ferri G, de Palma C, Canu T, Giovarelli M, Marra P, Fiocchi A, Molineris I, Raso M, Sanvito F, Doglioni C, Esposito A, Clementi E, Gabellini D (2019) The Suv420h histone methyltransferases regulate PPAR-γ and energy expenditure in response to environmental stimuli. Sci Adv 5:eaav1472.
Pambianco 2016 Cell Rep2016Pambianco S, Giovarelli M, Perrotta C, Zecchini S, Cervia D3, Di Renzo I, Moscheni C, Ripolone M, Violano R, Moggio M, Bassi MT, Puri PL, Latella L, Clementi E, De Palma C (2016) Reversal of defective mitochondrial biogenesis in limb-girdle muscular dystrophy 2D by independent modulation of histone and PGC-1α acetylation. Cell Rep 17:3010-23.

Abstracts

Add abstract

MitoEAGLE Short-Term Scientific Mission

Work Plan summary
1. Aim and motivation: At present my lab (Pharmacology Lab-University of Milan) is working on skeletal muscle physiology and bioenergetics in the context of muscular dystrophies (with murine models, e.g. alpha sarcoglycan KO and mdx mice) and mitochondria-related muscle phenotypes (Drp1 overexpression and mitochondrial dynamics).
Currently we are using two specific (standard) protocols with our O2k oxygraph for High Resolution Respirometry analyses using either permeabilized skeletal muscle bundles (from Tibialis Anterior, EDL, Gastrocnemius, Soleus, Quadricep and Diaphragm) or isolated mitochondria. Since the huge number of HRR protocols published over the years, the aim of my STSM consists in comparing my protocols with those used in the host lab in order to harmonize our procedures and, accordingly, to match experimental results. Another aim of my experience is, on the one hand, to share my expertise with the host lab, improving my personal skills, on the other hand to create a productive collaboration and to strengthen the relationship within MitoEAGLE network.
2. Proposed contribution to the scientific objectives of the Action: This project is in line with MitoEAGLE action to improve mitochondrial function knowledge and to share protocols and data within firstly the members and then with scientific community; in particular our aim is the interlaboratory comparisons of specific protocols, a critical issue in results interpretation.
3. Techniques: The project is based on the use of Oroboros O2k oxygraph for High Resolution Respirometry of cells and skeletal muscle fibers.
4. Planning: The one-month-plan shall include firstly a proficiency test (PT) including an instrumental background and an experiment (reference protocol RP1 and RP2) in HEK cells in order to access to host lab. Following the analysis step, I will work with permeabilized fibers; the plan is to prepare fibers and run a SUIT protocol in use in host lab with results in soleus to compare the fiber preparation. Then I will perform HRR experiments with the protocol for permeabilized fibers in use in my lab in Milan (same person, same protocol and sample in different labs) and then follow the protocol of host lab to see if we obtain the same results. This can fit in the generation of more data (interlaboratory comparisons) for muscle and it can be compare with data from other users. Last week has to be reserved for data analysis, make a report and present the results obtained.

Participated at


Visiting scientist in the Oroboros MitoFit Laboratory

O2k-Network

Matteo Giovarelli : Visiting scientist at the Oroboros MitoFit Laboratory

  • Feburary 05 to March 02