Gellerich 2002 Biochim Biophys Acta (Vol 1554)

From Bioblast
Publications in the MiPMap
Gellerich FN, Laterveer FD, Zierz S, Nicolay K (2002) The quantitation of ADP diffusion gradients across the outer membrane of heart mitochondria in the presence of macromolecules. Biochim Biophys Acta 1554:48-56.

Β» PMID: 12034470

Gellerich FN, Laterveer FD, Zierz S, Nicolay K (2002) Biochim Biophys Acta

Abstract: We have previously provided evidence that diffusion of metabolites across the porin pores of mitochondrial outer membrane is hindered. A functional consequence of this diffusion limitation is the dynamic compartmentation of ADP in the intermembrane space. These earlier studies were done on isolated mitochondria suspended in isotonic media without macromolecules, in which intermembrane space of mitochondria is enlarged. The present study was undertaken to assess the diffusion limitation of outer membrane in the presence of 10% (w/v) dextran M20, in order to mimick the action of cytosolic macromolecules on mitochondria. Under these conditions, mitochondria have a more native, condensed configuration.

Flux-dependent concentration gradients of ADP were estimated by measuring the ADP diffusion fluxes across the porin pores of isolated rat heart mitochondria incubated together with pyruvate kinase (PK), both of which compete for ADP regenerated by mitochondrial creatine kinase (mtCK) within the intermembrane space or by yeast hexokinase (HK) extramitochondrially. From diffusion fluxes and bulk phase concentrations of ADP, its concentrations in the intermembrane space were calculated using Fick's law of diffusion. Flux-dependent gradients up to 23 ΞΌM ADP (for a diffusion rate of JDif=1.9 ΞΌmol ADP/min/mg mitochondrial protein) were observed. These gradients are about twice those estimated in the absence of dextran and in the same order of magnitude as the cytosolic ADP concentration (30 ΞΌM), but they are negligibly low for cytosolic ATP (5 mM). Therefore, it is concluded that the dynamic ADP compartmentation is of biological importance for intact heart cells.

If mtCK generates ADP within the intermembrane space, the local ADP concentration can be clearly higher than in the cytosol resulting in higher extramitochondrial phosphorylation potentials. In this way, mtCK contributes to ensure optimal kinetic conditions for ATP-splitting reactions in the extramitochondrial compartment. β€’ Keywords: Macromolecule, Oxidative phosphorylation, Creatine kinase; Hexokinase, Compartmentation, Concentration gradient, Rat

β€’ O2k-Network Lab: NL Eindhoven Nicolay K, DE Magdeburg Gellerich FN, AT Innsbruck Oroboros


Organism: Rat  Tissue;cell: Heart  Preparation: Isolated mitochondria  Enzyme: Inner mt-membrane transporter 

Coupling state: OXPHOS 

HRR: Oxygraph-2k 

Cookies help us deliver our services. By using our services, you agree to our use of cookies.