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Garcia-Roche 2018 Redox Biol

From Bioblast
Publications in the MiPMap
García-Roche M, Casal A, Carriquiry M, Radi R, Celia Quijano C, Cassina A (2018) Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies. Redox Biol 17:207-12.

» Open Access

Garcia-Roche M, Casal A, Carriquiry M, Radi R, Celia Quijano C, Cassina A (2018) Redox Biol

Abstract: The aim of this work was to develop a cryopreservation method of small liver biopsies for in situ mitochondrial function assessment. Herein we describe a detailed protocol for tissue collection, cryopreservation, high-resolution respirometry using complex I and II substrates, calculation and interpretation of respiratory parameters. Liver biopsies from cow and rat were sequentially frozen in a medium containing dimethylsulfoxide as cryoprotectant and stored for up to 3 months at -80°C. Oxygen consumption rate studies of fresh and cryopreserved samples revealed that most respiratory parameters remained unchanged. Additionally, outer mitochondrial membrane integrity was assessed adding cytochrome c, proving that our cryopreservation method does not harm mitochondrial structure. In sum, we present a reliable way to cryopreserve small liver biopsies without affecting mitochondrial function. Our protocol will enable the transport and storage of samples, extending and facilitating mitochondrial function analysis of liver biopsies.

Bioblast editor: Kandolf G O2k-Network Lab: UY Montevideo Radi R


Labels: MiParea: Respiration 

Stress:Cryopreservation  Organism: Rat, Bovines  Tissue;cell: Liver  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 

2018-04