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Ferrera 2015 J Thorac Cardiovasc Surg

From Bioblast
Publications in the MiPMap
Ferrera R, Benhabbouche S, Da Silva CC, Alam MR, Ovize M (2015) Delayed low pressure at reperfusion: A new approach for cardioprotection. J Thorac Cardiovasc Surg 150:1641-8.

Β» PMID: 26384749

Ferrera R, Benhabbouche S, Da Silva CC, Alam MR, Ovize M (2015) J Thorac Cardiovasc Surg

Abstract: The aims of this study were to evaluate whether the delayed application of low-pressure reperfusion could reduce lethal reperfusion injury and whether the inhibition of the opening of the mitochondrial permeability transition pore is involved in this protection.

Isolated rat hearts (n = 120) underwent 40 minutes of global ischemia followed by 60 minutes of reperfusion. Hearts were randomly assigned to the following groups: control, postconditioning (comprising 2 episodes of 30 seconds of ischemia and 30 seconds of reperfusion), and low-pressure reperfusion (using a reduction of perfusion pressure at 70 cm H2O for 10 minutes). In additional groups, postconditioning and low-pressure reperfusion were applied after a delay of 3, 10, and 20 minutes after the initial 40-minute ischemic insult.

As expected, infarct size (triphenyltetrazolium chloride staining) and lactate dehydrogenase release were significantly reduced in low-pressure reperfusion and postconditioning versus controls (P < .01), whereas functional parameters (coronary flow, rate pressure product) were improved (P < .01). Although delaying postconditioning by more than 3 minutes resulted in a loss of protection, low-pressure reperfusion still significantly reduced infarct size when applied as late as 20 minutes after reperfusion. This delayed low-pressure reperfusion protection was associated with an improved mitochondrial respiration, lower reactive oxygen species production, and enhanced calcium retention capacity, related to inhibition of permeability transition pore opening.

We demonstrated for the first time that low-pressure reperfusion can reduce lethal myocardial reperfusion injury even when performed 10 to 20 minutes after the initiation of reperfusion. β€’ Keywords: Controlled reperfusion, Delayed reperfusion, Mitochondria, Permeability transition pore, Postconditioning

β€’ O2k-Network Lab: FR Lyon Ovize M

Labels: MiParea: Respiration 

Stress:Ischemia-reperfusion, Permeability transition  Organism: Rat  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS  Pathway:HRR: Oxygraph-2k 

2016-03, AmR