De Carvalho 2017 Sci Rep

From Bioblast
Publications in the MiPMap
de Carvalho AETS, Bassaneze V, Forni MF, Keusseyan AA, Kowaltowski AJ, Krieger JE (2017) Early postnatal cardiomyocyte proliferation requires high oxidative energy metabolism. Sci Rep 7:15434.

Β» PMID: 29133820 Open Access

de Carvalho AETS, Bassaneze V, Forni MF, Keusseyan AA, Kowaltowski AJ, Krieger JE (2017) Sci Rep

Abstract: Cardiac energy metabolism must cope with early postnatal changes in tissue oxygen tensions, hemodynamics, and cell proliferation to sustain development. Here, we tested the hypothesis that proliferating neonatal cardiomyocytes are dependent on high oxidative energy metabolism. We show that energy-related gene expression does not correlate with functional oxidative measurements in the developing heart. Gene expression analysis suggests a gradual overall upregulation of oxidative-related genes and pathways, whereas functional assessment in both cardiac tissue and cultured cardiomyocytes indicated that oxidative metabolism decreases between the first and seventh days after birth. Cardiomyocyte extracellular flux analysis indicated that the decrease in oxidative metabolism between the first and seventh days after birth was mostly related to lower rates of ATP-linked mitochondrial respiration, suggesting that overall energetic demands decrease during this period. In parallel, the proliferation rate was higher for early cardiomyocytes. Furthermore, in vitro nonlethal chemical inhibition of mitochondrial respiration reduced the proliferative capacity of early cardiomyocytes, indicating a high energy demand to sustain cardiomyocyte proliferation. Altogether, we provide evidence that early postnatal cardiomyocyte proliferative capacity correlates with high oxidative energy metabolism. The energy requirement decreases as the proliferation ceases in the following days, and both oxidative-dependent metabolism and anaerobic glycolysis subside.

β€’ Bioblast editor: Kandolf G β€’ O2k-Network Lab: BR Sao Paulo Kowaltowski AJ

Labels: MiParea: Respiration 

Organism: Rat  Tissue;cell: Heart  Preparation: Intact cells 

Coupling state: ROUTINE 

HRR: Oxygraph-2k 

Labels, 2018-01 

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