Dallon 2017 Biochem J
|Dallon BW, Parker BA, Hodson AE, Tippetts TS, Harrison ME, Appiah MMA, Witt JE, Gibbs JL, Gray HM, Sant TM, Bikman BT (2017) Insulin selectively reduces mitochondrial uncoupling in brown adipose tissue in mice. Biochem J 475:561-9.|
Abstract: The purpose of this study was to determine the effects of prolonged hyperinsulinemia on mitochondrial respiration and uncoupling in distinct adipose tissue depots.
Sixteen-wk-old male mice were injected daily with placebo or insulin to induce an artificial hyperinsulinemia for 28 d. Following treatment period, mitochondrial respiration and degree of uncoupling was determined in permeabilized perirenal, inguinal, and interscapular adipose tissue.
White adipose tissue (WAT) mitochondria (inguinal and perirenal) respire at substantially lower rates compared with brown adipose tissue (BAT). Insulin treatment resulted in a significant reduction in mitochondrial respiration in inguinal WAT (iWAT) and interscapular BAT (iBAT), but not perirenal WAT (pWAT). Furthermore, these changes were accompanied by an insulin-induced reduction in UCP1 and PGC1a in iWAT and iBAT only, but not in pWAT or skeletal muscle.
Compared to adipose tissue mitochondria in placebo conditions, adipose tissue from hyperinsulinemic mice manifested a site-specific reduction in mitochondrial respiration likely as a result of reduced uncoupling. These results may help explain weight gain so commonly seen with insulin treatment in type 2 diabetes mellitus.
Labels: MiParea: Respiration Pathology: Diabetes
Organism: Mouse Tissue;cell: Skeletal muscle, Fat Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET Pathway: N, NS, ROX HRR: Oxygraph-2k