Cunha de Padua 2017 Carbohydr Polym
Cunha de Padua MM, Suter Correia Cadena SM, de Oliveira Petkowicz CL, Martinez GR, Rodrigues Noleto G (2017) Toxicity of native and oxovanadium (IV/V) galactomannan complexes on HepG2 cells is related to impairment of mitochondrial functions. Carbohydr Polym 173:665-75. |
Cunha de Padua MM, Suter Correia Cadena SM, de Oliveira Petkowicz CL, Martinez GR, Rodrigues Noleto G (2017) Carbohydr Polym
Abstract: Polysaccharides and vanadium compounds have been studied due to their antitumor potential. In this study, the cytotoxic effects of galactomannan preparations on HepG2 cells were investigated. Native galactomannan from S. amazonicum (SAGM) and its modified form (MSAGM) were complexed with oxovanadium resulting in SAGM:VO and MSAGM:VO, respectively. The complexation was confirmed by NMR, FTIR, and AAS. SAGM and MSAGM:VO (250ΞΌg/mL) after 72h decreased viability by 51% and 58%, respectively, while the inhibition of the HepG2 cell proliferation was of βΌ27% and βΌ46%, respectively. SAGM and MSAGM:VO (250ΞΌg/mL) significantly inhibited all states of respiration (basal: 85% and 63%; uncoupled: 90% and 70%; and leak: 30% and 58%) after 72h. ROS levels increased by βΌ149% after the treatment with MSAGM:VO (250ΞΌg/mL) for 72h, while ΞΞ¨m decreased by βΌ50%. Our results indicate that galactomannan preparations from S. amazonicum, especially SAGM and the MSAGM:VO complex, could be considered as potential antitumor drugs for further investigations, once they have the ability to make HepG2 cells susceptible to death by affecting vital cellular processes such as respiration and ROS generation.
Copyright Β© 2017 Elsevier Ltd. All rights reserved. β’ Keywords: Antitumor, Cytotoxic effects, Galactomannans, HepG2 cells, Oxovanadium β’ Bioblast editor: Kandolf G
Labels: MiParea: Respiration, Pharmacology;toxicology
Pathology: Cancer
Organism: Human Tissue;cell: Liver
Coupling state: LEAK, ROUTINE, ET Pathway: ROX HRR: Oxygraph-2k
Labels, 2017-08