Cruz 2020 Cancers (Basel)
| Cruz B, Oliveira A, Viana LR, Lopes-Aguiar L, Canevarolo R, Colombera MC, Valentim RR, Garcia-Fóssa F, de Sousa LM, Castelucci BG, Consonni SR, Martins-de-Souza D, de Jesus MB, Russell ST, Gomes-Mardondes MCC (2020) Leucine-tich diet modulates the metabolomic and proteomic profile of skeletal muscle during cancer cachexia. Cancers (Basel) 12:E1880. |
Cruz Bread, Oliveira Andre, Viana Lais Rosa, Lopes-Aguiar Leisa, Canevarolo Rafael, Colombera Maiara Caroline, Valentim Rafael Rossi, Garcia-Fossa Fernanda, de Sousa Lizandra Maia, Castelucci Bianca Gazieri, Consonni Silvio Roberto, Martins-de-Souza Daniel, de Jesus Marcelo Bispo, Russell Steven Thomas, Cintra Gomes-Mardondes Maria Cristina (2020) Cancers (Basel)
Abstract: Cancer-cachexia induces a variety of metabolic disorders, including skeletal muscle imbalance. Alternative therapy, as nutritional supplementation with leucine, shows a modulatory effect over tumour damage in vivo and in vitro.
Adult rats distributed into Control (C), Walker tumour-bearing (W), control fed a leucine-rich diet (L), and tumour-bearing fed a leucine-rich diet (WL) groups had the gastrocnemius muscle metabolomic and proteomic assays performed in parallel to in vitro assays.
W group presented an affected muscle metabolomic and proteomic profile mainly related to energy generation and carbohydrates catabolic processes, but leucine-supplemented group (WL) recovered the energy production. In vitro assay showed that cell proliferation, mitochondria number and oxygen consumption were higher under leucine effect than the tumour influence. Muscle proteomics results showed that the main affected cell component was mitochondria, leading to an impacted energy generation, including impairment in proteins of the tricarboxylic cycle and carbohydrates catabolic processes, which were modulated and improved by leucine treatment.
In summary, we showed a beneficial effect of leucine upon mitochondria, providing information about the muscle glycolytic pathways used by this amino acid, where it can be associated with the preservation of morphometric parameters and consequent protection against the effects of cachexia. • Keywords: Experimental cachexia, Leucine-rich diet, Metabolomic, Mitochondria dysfunction, Proteomic, Skeletal muscle • Bioblast editor: Plangger M
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Pathology: Cancer
Organism: Mouse Tissue;cell: Skeletal muscle Preparation: Intact cells
Coupling state: LEAK, ROUTINE, ET
HRR: Oxygraph-2k
2020-07
