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Chang 2020 J Biol Chem

From Bioblast
Publications in the MiPMap
Chang L, Liu X, Diggle CP, Boyle JP, Hopkins PM, Shaw MA, Allen PD (2020) Bioenergetic defects in muscle fibers of RYR1 mutant knock-in mice associated with malignant hyperthermia. J Biol Chem 295:15226-35.

Β» PMID: 32826313 Open Access

Chang Leon, Liu Xiaochen, Diggle Christine P, Boyle John P, Hopkins Philip M, Shaw Marie-Anne, Allen Paul D (2020) J Biol Chem

Abstract: Mutations in the skeletal muscle ryanodine receptor gene (RYR1) can cause susceptibility to malignant hyperthermia (MH), a potentially lethal genetic condition triggered by volatile anesthetics. MH is associated with hypermetabolism which has directed research interest into oxidative phosphorylation (OXPHOS) and muscle bioenergetics. The most common cause of MH in the United Kingdom is the c.7300G>A RYR1 variant, which is present in ~16% of MH families. Our study focuses on the MH susceptible G2435R-RYR1 knock-in mouse model, which is the murine equivalent of the human c.7300G>A genotype. Using a combination of transcriptomics, protein expression and functional analysis, we investigated adult muscle fiber bioenergetics in this mouse model. RNA sequencing data showed reduced expression of genes associated with mitochondria and fatty acid oxidation in RYR1 mutants when compared to wild-type (WT) controls. Mitochondrial function was assessed by measuring oxygen consumption rates in permeabilized muscle fibers. Comparisons between WT and homozygous G2435R-RYR1 mitochondria showed a significant increase in complex I-facilitated OXPHOS in mutant muscle. Furthermore, we observed a gene-dose specific increase in reactive oxygen species production in G2435R-RYR1 muscle fibers. Collectively these findings provide evidence of metabolic defects in G2435R-RYR1 knock-in mouse muscle under basal conditions. Differences in metabolic profile could be the result of differential gene expression in metabolic pathways, in conjunction with mitochondrial damage accumulated from chronic exposure to increased oxidative stress. β€’ Keywords: Bioenergetics, Malignant hyperthermia, Mitochondrial metabolism, Mouse, Muscle physiology, Ryanodine receptor β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: UK Leeds Peers C

Labels: MiParea: Respiration, nDNA;cell genetics, Genetic knockout;overexpression 

Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k