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Casanova 2014 J Nutr Biochem

From Bioblast
Publications in the MiPMap
Casanova E, Baselga-Escudero L, Ribas-Latre A, Cedó L, Arola-Arnal A, Pinent M, Bladé C, Arola L, Salvadó MJ (2014) Chronic intake of proanthocyanidins and docosahexaenoic acid improves skeletal muscle oxidative capacity in diet-obese rats. J Nutr Biochem 25:1003–10.


Casanova E, Baselga-Escudero L, Ribas-Latre A, Cedo L, Arola-Arnal A, Pinent M, Blade C, Arola L, Salvado MJ (2014) J Nutr Biochem

Abstract: Obesity has become a worldwide epidemic. The cafeteria diet (CD) induces obesity and oxidative stress-associated insulin resistance. Polyunsaturated fatty acids and polyphenols are dietary compounds that are intensively studied as products that can reduce the health complications related to obesity. We evaluate the effects of 21days of supplementation with grape seed proanthocyanidins extract (GSPE), docosahexaenoic-rich oil (DHA-OR) or both compounds (GSPE+DHA-OR) on skeletal muscle metabolism in diet-obese rats. The supplementation with different treatments did not reduce body weight although all groups used more fat as fuel, particularly when both products were co-administered; muscle β-oxidation was activated, the mitochondrial functionality and oxidative capacity was higher, and fatty acid uptake gene expressions were upregulated. In addition to these outcomes shared by all treatments, GSPE reduced insulin resistance, and improved muscle status. Both treatments increased 5’-AMP-activated protein kinase (AMPK) phosphorylation, which was consistent with higher plasma adiponectin levels. Moreover AMPK activation by DHA-OR was also correlated with an upregulation of peroxisome proliferator-activated receptor alpha (Pparα). GSPE+DHA-OR, in addition to activate AMPK and enhance fatty acid oxidation, increased the muscle gene expression of uncoupling protein 2 (Ucp2). In conclusion GSPE+DHA-OR induced modifications that improved muscle status and could counterbalance the deleterious effects of obesity, and such modifications are mediated at least in part, through the AMPK signaling pathway. Keywords: Obesity, Docosahexaenoic acid, Proanthocyanidins, Skeletal muscle, Mitochondria, β-oxidation

O2k-Network Lab: ES Tarragona Arola L

Labels: MiParea: Respiration  Pathology: Obesity 

Organism: Rat  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS  HRR: Oxygraph-2k