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Cannon 2016 Lung

From Bioblast
Publications in the MiPMap
Cannon DT, Liu J, Sakurai R, Rossiter HB, Rehan VK (2016) Impaired lung mitochondrial respiration following perinatal nicotine exposure in rats. Lung 194:325-8.

» PMID: 26899624

Cannon DT, Liu J, Sakurai R, Rossiter HB, Rehan VK (2016) Lung

Abstract: Perinatal smoke/nicotine exposure predisposes to chronic lung disease and morbidity. Mitochondrial abnormalities may contribute as the PPARγ pathway is involved in structural and functional airway deficits after perinatal nicotine exposure. We hypothesized perinatal nicotine exposure results in lung mitochondrial dysfunction that can be rescued by rosiglitazone (RGZ; PPARγ receptor agonist). Sprague-Dawley dams received placebo (CON), nicotine (NIC, 1 mg kg(-1)), or NIC + RGZ (3 mg kg(-1)) daily from embryonic day 6 to postnatal day 21. Parenchymal lung (~10 mg) was taken from adult male offspring for mitochondrial assessment in situ. ADP-stimulated O2 consumption was less in NIC and NIC + RGZ compared to CON (F[2,14] = 17.8; 4.5 ± 0.8 and 4.1 ± 1.4 vs. 8.8 ± 2.5 pmol s mg(-1); p < 0.05). The respiratory control ratio for ADP, an index of mitochondrial coupling, was reduced in NIC and remediated in NIC + RGZ (F[2,14] = 3.8; p < 0.05). Reduced mitochondrial oxidative capacity and abnormal coupling were evident after perinatal nicotine exposure. RGZ improved mitochondrial function through tighter coupling of oxidative phosphorylation.


O2k-Network Lab: US CA San Diego Cannon DT, US CA Torrance Rossiter HB


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Pharmacology;toxicology 


Organism: Rat  Tissue;cell: Lung;gill  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, CIV, NS  HRR: Oxygraph-2k 

2016-03