Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

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Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

Cannon DT (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum


  • Saponin permeabilized skeletal muscle fiber bundles
  • Protocol:
  1. 0.5mM Malate
  2. 50 µM palmitoyl-CoA + 2mM carnitine
  3. 5mM ADP
  4. From here on out, various combinations for titration protocol
  • Coupling states:
  1. LEAK_M+Palmitoylcarnitine
  2. P_M+Palmitoylcarnitine
  3. P_M+Palmitoylcarnitine+S
  4. E_O+CCCP titrations
  5. E_S+Rot
  6. ROX_AntimycinA



O2k-Network Lab: US CA San Diego Cannon DT


Labels:


Organism: Human, Mouse  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S, NS, ROX 


Palmitoylcarnitine