Brunner 2024 Life Sci Alliance
| Brunner S, Höring M, Liebisch G, Schweizer S, Scheiber J, Giansanti P, Hidrobo M, Hermeling S, Oeckl J, Prudente de Mello N, Perocchi F, Seeliger C, Strohmeyer A, Klingenspor M, Plagge J, Küster B, Burkhardt R, Janssen KP, Ecker J (2024) Mitochondrial lipidomes are tissue specific - low cholesterol contents relate to UCP1 activity. Life Sci Alliance 7:e202402828. https://doi.org/10.26508/lsa.202402828 |
Brunner Sarah, Hoering Marcus, Liebisch Gerhard, Schweizer Sabine, Scheiber Josef, Giansanti Piero, Hidrobo Maria, Hermeling Sven, Oeckl Josef, Prudente de Mello Natalia, Perocchi Fabiana, Seeliger Claudine, Strohmeyer Akim, Klingenspor Martin, Plagge Johannes, Kuester Bernhard, Burkhardt Ralph, Janssen Klaus-Peter, Ecker Josef (2024) Life Sci Alliance
Abstract: Lipid composition is conserved within sub-cellular compartments to maintain cell function. Lipidomic analyses of liver, muscle, white and brown adipose tissue (BAT) mitochondria revealed substantial differences in their glycerophospholipid (GPL) and free cholesterol (FC) contents. The GPL to FC ratio was 50-fold higher in brown than white adipose tissue mitochondria. Their purity was verified by comparison of proteomes with ER and mitochondria-associated membranes. A lipid signature containing PC and FC, calculated from the lipidomic profiles, allowed differentiation of mitochondria from BAT of mice housed at different temperatures. Elevating FC in BAT mitochondria prevented uncoupling protein (UCP) 1 function, whereas increasing GPL boosted it. Similarly, STARD3 overexpression facilitating mitochondrial FC import inhibited UCP1 function in primary brown adipocytes, whereas a knockdown promoted it. We conclude that the mitochondrial GPL/FC ratio is key for BAT function and propose that targeting it might be a promising strategy to promote UCP1 activity.
• Bioblast editor: Plangger M • O2k-Network Lab: DE Freising Klingenspor M
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Fat
Preparation: Isolated mitochondria
Regulation: Uncoupler Coupling state: ET, LEAK Pathway: N, Gp, ROX HRR: Oxygraph-2k
2024-07
