Bokhari 2021 Commun Biol
| Bokhari MH, Halleskog C, Γ slund A, Boulet N, CasadesΓΊs Rendos E, de Jong JMA, Csikasz R, Amri EZ, Shabalina I, Bengtsson T (2021) Isothermal microcalorimetry measures UCP1-mediated thermogenesis in mature brite adipocytes. Commun Biol 4:1108. |
Bokhari Muhammad Hamza, Halleskog Carina, Alice Aaslund Alice, Boulet Nathalie, Casadesus Rendos Eva, de Jong Jasper Martin Antont, Csikasz Robert, Amri Ez-Zoubir, Shabalina Irina, Bengtsson Tore (2021) Commun Biol
Abstract: The activation of thermogenesis in adipose tissue has emerged as an important target for the development of novel anti-obesity therapies. Using multi-well isothermal microcalorimetry, we have demonstrated that mature murine brown and brite adipocytes produce quantifiable heat upon Ξ²3-AR stimulation, independently of any anaerobic mechanisms. Additionally, in brite adipocytes lacking UCP1 protein, Ξ²3-AR stimulation still induces heat production, albeit to a much lower extent than in their wildtype counterparts, suggesting that UCP1 is an essential component of adrenergic induced thermogenesis in murine brite adipocytes exvivo. Similarly, we could observe an increase in heat production in human-derived adipocytes (hMADS) upon Ξ²-AR stimulation. Collectively, these results establish the use of isothermal microcalorimetry as a sensitive and accurate technique for measuring thermogenic responses in intact mature brite adipocytes from murine and human origin.
β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Fat
Coupling state: LEAK, ET
HRR: Oxygraph-2k
2021-10
