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Bokhari 2021 Commun Biol

From Bioblast
Publications in the MiPMap
Bokhari MH, Halleskog C, Åslund A, Boulet N, Casadesús Rendos E, de Jong JMA, Csikasz R, Amri EZ, Shabalina I, Bengtsson T (2021) Isothermal microcalorimetry measures UCP1-mediated thermogenesis in mature brite adipocytes. Commun Biol 4:1108.

» PMID: 34548622 Open Access

Bokhari Muhammad Hamza, Halleskog Carina, Alice Aaslund Alice, Boulet Nathalie, Casadesus Rendos Eva, de Jong Jasper Martin Antont, Csikasz Robert, Amri Ez-Zoubir, Shabalina Irina, Bengtsson Tore (2021) Commun Biol

Abstract: The activation of thermogenesis in adipose tissue has emerged as an important target for the development of novel anti-obesity therapies. Using multi-well isothermal microcalorimetry, we have demonstrated that mature murine brown and brite adipocytes produce quantifiable heat upon β3-AR stimulation, independently of any anaerobic mechanisms. Additionally, in brite adipocytes lacking UCP1 protein, β3-AR stimulation still induces heat production, albeit to a much lower extent than in their wildtype counterparts, suggesting that UCP1 is an essential component of adrenergic induced thermogenesis in murine brite adipocytes exvivo. Similarly, we could observe an increase in heat production in human-derived adipocytes (hMADS) upon β-AR stimulation. Collectively, these results establish the use of isothermal microcalorimetry as a sensitive and accurate technique for measuring thermogenic responses in intact mature brite adipocytes from murine and human origin.

Bioblast editor: Plangger M

Labels: MiParea: Respiration 

Organism: Mouse  Tissue;cell: Fat 

Coupling state: LEAK, ET 

HRR: Oxygraph-2k