Benayada 2024 J Funct Foods
| Benayada L, Gubitosa F, Fraternale D, Carloni S, Cerioni L, Matacchione G, Olivieri F, Potenza L, De Bellis R, Chiarantini L, Roselli C, Valentini L, Gobbi P, Balduini W, Pappagallo N, Ventura N, Kass GEN, Colomba M, Albertini MC (2024) In vitro anti-inflammatory activity of Malus Γ domestica var. Mela Rosa Marchigiana pulp callus extract contrasting high glucose conditions. J Funct Foods 121:106420. https://doi.org/10.1016/j.jff.2024.106420 |
Benayada Leila, Gubitosa Frederica, Fraternale Daniele, Carloni Silvia, Cerioni Liana, Matacchione Giulia, Olivieri Fabiola, Potenza Lucia, De Bellis Roberta, Chiarantini Laura, Roselli Carla, Valentini Laura, Gobbi Pietro, Balduini Walter, Pappagallo Noemi, Ventura Natascia, Kass George EN, Colomba Mariastella, Albertini Maria Cristina (2024) J Funct Foods
Abstract: The anti-inflammatory activity of the βMela Rosa Marchigianaβ (MRM) pulp callus ethanol Extract (MRME) was tested in different cellular models including (i) LPS-treated RAW 264.7; (ii) HUVEC exposed to short-term high Glucose (HG, 45 mM) or normal glucose (NG, 5 mM) concentrations; and (iii) HG (30 mN) and LPS-treated U937. To evaluate the anti-inflammatory effect of the extract, Nitric Oxide (NO) production was measured in RAW 264.6 cells, while IL-8, IL-1Γ and MCP-1 expressions, along with modulation of some inflammation- or senescence-associated miRNAs (miR-21, miR-126, miR-17 and miR-217) were assessed in HUVECs and/or U937. MRME treatment reduced pro-inflammatory markers amount, suggesting a decreased generalised inflammatory response. Present findings indicate that MRME can contrast senescence- or HG-induced inflammation. Moreover, in HG- and LPS-induced inflammation, MRME reduced U937 monocyte activation by inhibiting mitochondrial respiration and inflammatory markers. Finally, the inhibitory potential of MRME on the digestive enzymes Ξ±-glucosidase, Ξ±-amylase and lipase activity was investigated. Our results support the idea that MRME has a positive effect on inhibition of endothelial/macrophage dysfunction under HG/senescence inflammatory conditions. Furthermore, the obtained results showed a modest inhibitory power of the extract (IC50 values: 2.98 Β± 0.24, 1.77 Β± 0.15, and 2.06 Β± 0.31 mg/ml, respectively), which, however, could be of some help in decreasing the absorption of glucose and triglycerides.
β’ Bioblast editor: Plangger M β’ O2k-Network Lab: IT Urbino Cerioni L
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Human
Tissue;cell: Other cell lines
Preparation: Intact cells
Coupling state: ET, LEAK, ROUTINE
Pathway: ROX
HRR: Oxygraph-2k
2024-08
