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Belosludtseva 2020 Bull Exp Biol Med

From Bioblast
Publications in the MiPMap
Belosludtseva NV, Talanov EY, Venediktova NI, Sharapov MG, Mironova GD, Belosludtsev KN (2020) Structural and functional features of Ca2+ transport systems in liver mitochondria of rats with experimental hyperthyroidism. Bull Exp Biol Med 169:224-28.

Β» PMID: 32654002

Belosludtseva NV, Talanov EY, Venediktova NI, Sharapov MG, Mironova GD, Belosludtsev KN (2020) Bull Exp Biol Med

Abstract: We analyzed structural and functional features of the main mitochondrial Ca2+-transporting systems, mitochondrial Ca2+ uniporter complex (MCUC) and Ca2+-dependent cyclosporin A-sensitive mitochondrial permeability transition pore (MPT pore), in rats with hyperthyroid state. It was found that, the rate of Ca2+ accumulation by rat liver mitochondria in this pathology increases by 1.3 times, which can be associated with higher level of the channel-forming subunit of the uniporter MCU and lower content of dominant-negative subunit of this complex MCUb. At the same time, the level of the regulatory subunit MICU1 remained unchanged. It was shown that calcium retention capacity of liver mitochondria in rats with experimental hyperthyroidism decreased by 2 times in comparison with the control, which attested to reduced resistance of liver mitochondria of hyperthyroid rats to induction of the MPT pore. The observed changes are consistent with the data on increased amount of cyclophilin D, a mitochondrial matrix peptidyl-prolyl isomerase that is known to modulate the MPT pore opening and expression of the Ppif gene that encodes mitochondrial cyclophilin D in rats with experimental hyperthyroidism. β€’ Keywords: Ca2+-induced mitochondrial permeability transition pore, Cyclophilin D, Hyperthyroidism, Mitochondria, Mitochondrial Ca2+ uniporter β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: RU Pushchino Mironova GD


Labels: MiParea: Respiration  Pathology: Other  Stress:Permeability transition  Organism: Rat  Tissue;cell: Liver  Preparation: Isolated mitochondria 

Regulation: Calcium, Ion;substrate transport 


HRR: Oxygraph-2k 

2020-07