 |
Flux baseline correction |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
Flux baseline correction provides the option to display the plot and all values of the flux (or flow, or flux control ratio) as the total flux, J, minus a baseline flux, J0.
JV(bc) = JV - JV0 JV = (dc/dt) Β· Ξ½-1 Β· SF - JΒ°V
For the oxygen channel, JV is O2 flux per volume [pmol/(sΒ·ml)] (or volume-specific O2 flux), c is the oxygen concentration [nmol/ml = Β΅mol/l = Β΅M], dc/dt is the (positive) slope of oxygen concentration over time [nmol/(s Β· ml)], Ξ½-1 = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), SF=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and JΒ°V is the volume-specific background oxygen flux (Instrumental background oxygen flux). Further details: Flux / Slope.
Abbreviation: bc
MitoPedia methods:
Respirometry,
Fluorometry
MitoPedia O2k and high-resolution respirometry:
DatLab
