Barrientos 2009 Curr Protoc Hum Genet

From Bioblast
Jump to: navigation, search
Publications in the MiPMap
Barrientos A, Fontanesi F, Díaz F (2009) Evaluation of the mitochondrial respiratory chain and oxidative phosphorylation system using polarography and spectrophotometric enzyme assays. Curr Protoc Hum Genet Chapter 19:Unit19.3. doi: 10.1002/0471142905.hg1903s63.

» PMID: 19806590 Open Access

Barrientos A, Fontanesi F, Diaz F (2009) Curr Protoc Hum Genet

Abstract: The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial inner membrane. They work in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging. For this reason, several approaches for the assessment of the OXPHOS system enzymes have been developed. Based on the methods described elsewhere, the assays describe methods that form a biochemical characterization of the OXPHOS system in cells and mitochondria isolated from cultured cells or tissues.

Keywords: Electron transport chain, Mitochondria, OXPHOS, Polarography, Spectrophotometry


Labels: MiParea: Respiration, Instruments;methods 







Comments

>> Respiratory complexes - more than five