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Bakke 2012 Biochim Biophys Acta

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Bakke SS, Moro C, Nikolić N, Hessvik NP, Badin PM, Lauvhaug L, Fredriksson K, Hesselink MK, Boekschoten MV, Kersten S, Gaster M, Thoresen GH, Rustan AC (2012) Palmitic acid follows a different metabolic pathway than oleic acid in human skeletal muscle cells; lower lipolysis rate despite an increased level of adipose triglyceride lipase. Biochim Biophys Acta 1821:1323-33.

» PMID: 22796147

Bakke SS, Moro C, Nikolic N, Hessvik NP, Badin PM, Lauvhaug L, Fredriksson K, Hesselink MK, Boekschoten MV, Kersten S, Gaster M, Thoresen GH, Rustan AC (2012) Biochim Biophys Acta

Abstract: Development of insulin resistance is positively associated with dietary saturated fatty acids and negatively associated with monounsaturated fatty acids. To clarify aspects of this difference we have compared the metabolism of oleic (OA, monounsaturated) and palmitic acids (PA, saturated) in human myotubes. Human myotubes were treated with 100μM OA or PA and the metabolism of [(14)C]-labeled fatty acid was studied. We observed that PA had a lower lipolysis rate than OA, despite a more than two-fold higher protein level of adipose triglyceride lipase after 24h incubation with PA. PA was less incorporated into triacylglycerol and more incorporated into phospholipids after 24h. Supporting this, incubation with compounds modifying lipolysis and reesterification pathways suggested a less influenced PA than OA metabolism. In addition, PA showed a lower accumulation than OA, though PA was oxidized to a relatively higher extent than OA. Gene set enrichment analysis revealed that 24h of PA treatment upregulated lipogenesis and fatty acid β-oxidation and downregulated oxidative phosphorylation compared to OA. The differences in lipid accumulation and lipolysis between OA and PA were eliminated in combination with eicosapentaenoic acid (polyunsaturated fatty acid). In conclusion, this study reveals that the two most abundant fatty acids in our diet are partitioned toward different metabolic pathways in muscle cells, and this may be relevant to understand the link between dietary fat and skeletal muscle insulin resistance.

Copyright © 2012 Elsevier B.V. All rights reserved.


Labels: Pathology: Diabetes 

Organism: Human  Tissue;cell: Skeletal muscle