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Babylon 2022 Int J Mol Sci

From Bioblast
Publications in the MiPMap
Babylon L, Schmitt F, Franke Y, Hubert T, Eckert GP (2022) Effects of combining biofactors on bioenergetic parameters, Aβ levels and survival in Alzheimer model organisms.

» Int J Mol Sci 23:8670. PMID: 35955803 Open Access

Babylon Lukas, Schmitt Fabian, Franke Yannik, Hubert Tim, Eckert Gunter P (2022) Int J Mol Sci

Abstract: Increased amyloid beta (Aβ) levels and mitochondrial dysfunction (MD) in the human brain characterize Alzheimer disease (AD). Folic acid, magnesium and vitamin B6 are essential micro-nutrients that may provide neuroprotection. Bioenergetic parameters and amyloid precursor protein (APP) processing products were investigated in vitro in human neuroblastoma SH-SY5Y-APP695 cells, expressing neuronal APP, and in vivo, in the invertebrate Caenorhabditis elegans (CL2006 & GMC101) expressing muscular APP. Model organisms were incubated with either folic acid and magnesium-orotate (ID63) or folic acid, magnesium-orotate and vitamin B6 (ID64) in different concentrations. ID63 and ID64 reduced Aβ, soluble alpha APP (sAPPα), and lactate levels in SH-SY5Y-APP695 cells. The latter might be explained by enhanced expression of lactate dehydrogenase (LDHA). Micronutrient combinations had no effects on mitochondrial parameters in SH-SY5Y-APP695 cells. ID64 showed a significant life-prolonging effect in C. elegans CL2006. Incubation of GMC101 with ID63 significantly lowered Aβ aggregation. Both combinations significantly reduced paralysis and thus improved the phenotype in GMC101. Thus, the combinations of the tested biofactors are effective in pre-clinical models of AD by interfering with Aβ related pathways and glycolysis. Keywords: Alzheimer disease, C. elegans, Amyloid beta, Biofactor, Folic acid, Magnesium-orotate, Mitochondria, Mitochondria dysfunction, Vitamin B6 Bioblast editor: Plangger M O2k-Network Lab: DE Giessen Eckert GP

Labels: MiParea: Respiration  Pathology: Alzheimer's 

Organism: Human  Tissue;cell: Neuroblastoma  Preparation: Permeabilized cells 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k