Auranofin

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MitoPedia

Auranofin

Description

Auranofin (AF) is a gold complex which inhibites thioredoxin reductase (TrxR).

Abbreviation: AF






MitoPedia topics: Inhibitor 

Communicated by Doerrier Carolina 2017-04-05.

H2O2 production

Oxidative stress plays an important role in many pathological conditions, therefore mitochondrial (ROS) measurements are crucial for understanding a disease and for evaluating specific treatments. Evaluation of hydrogen peroxide (H2O2) emission flux by fluorescence can be used to detect H2O2 production in biological samples. Mitochondrial H2O2 emission flux is a balance between H2O2 generation, superoxide (O2•-) dismutation and the H2O2 scavenging. Mitochondrial antioxidant systems (which scavenge ROS) can be stimulated in specific physiopathological conditions masking the real ROS generation. Auranofin (AF) is an inhibitor of thioredoxin reductase (TrxR). AF can be used to quantify the relative contribution of one of the main antioxidant systems in mitochondria: Trx system. Additionally, dinitrochlorobenzene (DNCB), an inhibitor of glutathione (GSH) can be used to obtain the GSH contribution scavenging H2O2.



References

  1. Aon MA, Stanley AS, Sivakumaran V, Kembro JM, O´Rourke B, Paolocci N, Cortassa S (2012) Glutathione/thioredoxin systems modulate mitochondrial H2O2 emission: An experimental-computational study. J Gen Physiol 139(6):479-91.
  2. Hwang-Bo H, Jeong JW, Han MH, Park C, Hong SH, Kim GY, Moon SK, Cheong J, Kim WJ, Yoo YH, Choi YH (2017) Auranofin, an inhibitor of thioredoxin reductase, induces apoptosis in hepatocellular carcinoma Hep3B cells by generation of reactive oxygen species. Gen Physiol Biophys. 36(2):117-128. doi: 10.4149/gpb_2016043