Affourtit 2012 Methods Mol Biol
|Affourtit C, Quinlan CL, Brand MD (2012) Measurement of proton leak and electron leak in isolated mitochondria. Methods Mol Biol 810:165-82.|
Abstract: Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.
• Keywords: mitochondria, oxygen consumption, membrane potential, reactive oxygen species production, oxidative phosphorylation, modular kinetic analysis, proton leak, electron leak • Bioblast editor: Sumbalova Z
Labels: MiParea: Respiration
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Skeletal muscle
Enzyme: Complex II;succinate dehydrogenase, Complex III Regulation: mt-Membrane potential Coupling state: LEAK
HRR: TPP, Theory