Talk:MiPNet17.08 IOC68 Schroecken AT

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IOC68 Workshop Experiments

Oxygraph AB (in both chambers the same SUIT protocol was applied)
DatLab File 2012-04-14_AB-02
mouse heart homogenate prepared with PBI Shredder (position 1 for 10 seconds, position 2 for 5 seconds) and the homogenate was completely washed out of the Shredder tube with 5 ml MiR05
2.3 mg tissue per chamber = 1.15 mg / ml
Flux derivation = 20
oxygen regime 320 to 160 (reoxygenation with pure oxygen at open chamber position)
respiration medium: MiR05 (solubility factor = 0.92)
SUIT-Protocol: PMG + AmR + HRP + H₂O₂ + H₂O₂ + D2 + D3 + S + FCCP + Rot + H₂O₂ + H₂O₂ + Mna + Ama
the homogenate was added, the oxygen concentration was increased till 320 nmol/ml and then the chamber was closed

there is a dependence of oxygen with homogenate but not that strong as with fibres (decrease of oxygen flux with decrease of oxygen concentration)
stimulation of respiration with ADP and succinate
reoxygenation with pure oxygen causes an increase in peroxide production
no big effect of FCCP on oxygen flux but a decrease in peroxide production occurred
oxygen dependence visible in the decline of oxygen flux and NO signal
rotenone increased the peroxide production
analysis of the experiments is difficult because of O₂ dependence