Salicylhydroxamic acid: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14. | ::: '''SHAM: Salicylhydroxamic acid''' (99%; HOC<sub>6</sub>H<sub>4</sub>CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14. | ||
::::<span style="color:#8B008B"> '''Caution:''' Irritant!</span> | |||
::::'''Preparation of 20 mM stock solution (dissolved in DMSO)''': | |||
:::'''Preparation of 20 mM stock solution (dissolved in DMSO)''': | |||
::::# Weigh 6.1 mg of SHAM. | ::::# Weigh 6.1 mg of SHAM. | ||
::::# Dissolve in 2 mL of DMSO. | ::::# Dissolve in 2 mL of DMSO. | ||
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::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
::: '''O2k Manual Titrations:''' [[MiPNet09.12 O2k-Titrations]] | :::» '''O2k Manual Titrations:''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 100 µL | ::::* Titration volume: 100 µL | ||
Revision as of 10:35, 11 September 2020
- high-resolution terminology - matching measurements at high-resolution
Salicylhydroxamic acid
Description
Salicylhydroxamic acid (SHAM; synonym: 2-Hydroxybenzohydroxamic acid N,2-Dihydroxybenzamide) is an inhibitor of the alternative oxidase (AOX). When AOX is blocked by SHAM, electrons are forced through the CIII-cytochrome c oxidase pathway, allowing observation of the operation of the CIII-CIV pathway without AOX activity.
Abbreviation: SHAM
Reference: Parrino_2000_J_Exp_Biol
Application in HRR
- SHAM: Salicylhydroxamic acid (99%; HOC6H4CONHOH); Sigma S 607, 5 g, store at RT; FW = 153.14.
- Caution: Irritant!
- Preparation of 20 mM stock solution (dissolved in DMSO):
- Weigh 6.1 mg of SHAM.
- Dissolve in 2 mL of DMSO.
- Divide into 0.2 mL portions
- Store at -20 °C.
- Preparation of 20 mM stock solution (dissolved in DMSO):
- » O2k Manual Titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 100 µL
- Final conc. in 2 mL O2k chamber: 1 mM
MitoPedia topics:
Inhibitor
