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SUIT-018 O2 mt D054
Description
Abbreviation: NS(GM)
Reference: B: SUIT-018
SUIT number: D054_1GMS;2D;3Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: NS(GM)
- SUIT protocol pattern: lineal 1GMS;2D-
SUIT-018 O2 mt D054 is a short protocol to study the influence of a fluorescence dye on the O2 flux. During this protocol the [O2] can be modified injecting nitrogen in the chamber.
Communicated by Komlodi T and Gnaiger E (last update 2019-04-03)
Steps and respiratory states
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
1GMS
|
GMSL(n)
|
NS
|
CI&CII
|
1GMS
- Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
- Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
|
2D
|
GMSP
|
NS
|
CI&CII
|
1GMS;2D
- Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
2c
|
GMScP
|
NS
|
CI&CII
|
1GMS;2D;2c
- Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
- Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
|
3Ama
|
ROX
|
|
|
1GMS;2D;2c;3Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|
Step
|
Respiratory state
|
Pathway control
|
ET-Complex
|
Comment
|
## AsTm
|
AsTmE
|
CIV
|
CIV
|
|
## Azd
|
CHB
|
|
|
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- » Coupling control state
- » ET capacity
- » OXPHOS capacity
- » LEAK respiration
- Pathway control
- » Electron transfer pathway
- » Fatty acid oxidation pathway control state, F
- » NADH electron transfer-pathway state, N
- » Succinate pathway control state, S
- » NS-pathway control state, NS
- » Glycerophosphate pathway control state, Gp
- » Complex IV single step, CIV
- » Anaplerotic pathway control state
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Glossary
- » List of SUIT states
- » SUIT concept
Strengths and limitations
- + Reasonable duration of the experiment.
- - CIV activity and cytochrome c test can be performed.
Compare SUIT protocols
MitoPedia concepts:
SUIT protocol,
SUIT B,
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MitoPedia methods:
Respirometry