Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=ceS | ||
|description=[[File: | |description=[[File:ce1;ce2SD;ce3U;ce4Rot;ce5Ama.png|200px]] | ||
|info=[[Steinlechner-Maran 1997 Transplantation]] | |info='''B''' [[Steinlechner-Maran 1997 Transplantation]] | ||
|application=O2 | |||
}} | }} | ||
::: '''A''' Advantageous alternative protocol: [[SUIT-003]] | |||
::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling-control protocol]] (ceCCP) | |||
'''R;L;E format_ce1;ce2S;ce3U-''' | |||
::: '''[[ | |||
::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] ( | [[File:R;L;E format_1R;2S;3U-.jpg|left|200px]] | ||
== Cell coupling-control protocol == | |||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
| Line 23: | Line 32: | ||
|- | |- | ||
| | | ce1 | ||
| [[ROUTINE]] | | [[ROUTINE]] | ||
| [[ | | [[Living cells |ce]] | ||
| | | Living cells, ce, are in the ROUTINE state of respiration. Mitochondria of dead cells (dce) without substrate are in a state of ROX. | ||
|- | |- | ||
| | | ce2S | ||
| ''R''+S<sub>''L''</sub> | | ''R''+S<sub>''L''</sub> | ||
| [[ROUTINE]] (''R'') + [[S]] (S<sub>''L''</sub>) | | [[ROUTINE]] (''R'') + [[S]] (S<sub>''L''</sub>) | ||
| | | dce are in state S<sub>''L''</sub>. ADP should be added to stimulate mitochondria in dce to the OXPHOS state; see [[1ce;2ceSD;3ceU;4ceRot;5ceAma]]. | ||
|- | |- | ||
| | | ce3U | ||
| ''E''+S<sub>''E''</sub> | | ''E''+S<sub>''E''</sub> | ||
| [[ | | [[Electron transfer pathway]] (''E'') + [[S]] (S<sub>''E''</sub>) | ||
| ce and | | ce and dce are in the ET-pathway state. | ||
|- | |- | ||
| | | ce4Rot | ||
| ROX+S<sub>''E''</sub> | | ROX+S<sub>''E''</sub> | ||
| [[ROX]]+[[S]] (S<sub>''E''</sub>) | | [[ROX]]+[[S]] (S<sub>''E''</sub>) | ||
| ce are in the ROX state, | | ce are in the ROX state, dce are in the ET-pathway state. | ||
|- | |- | ||
| | | ce5Ama | ||
| ROX<sub>Ama</sub> | | ROX<sub>Ama</sub> | ||
| [[ROX]] | | [[ROX]] | ||
| Line 53: | Line 62: | ||
|} | |} | ||
== Compare SUIT protocols == | |||
::::* [[SUIT-003]] ('''A''' Advantageous alternative protocol) | |||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol, SUIT B | |||
}} | |||
Latest revision as of 09:44, 3 June 2020
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama
Description
Abbreviation: ceS
Reference: B Steinlechner-Maran 1997 Transplantation
O2k-Application: O2
- A Advantageous alternative protocol: SUIT-003
- SUIT protocol pattern: complex cell coupling-control protocol (ceCCP)
R;L;E format_ce1;ce2S;ce3U-
Cell coupling-control protocol
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| ce1 | ROUTINE | ce | Living cells, ce, are in the ROUTINE state of respiration. Mitochondria of dead cells (dce) without substrate are in a state of ROX. |
| ce2S | R+SL | ROUTINE (R) + S (SL) | dce are in state SL. ADP should be added to stimulate mitochondria in dce to the OXPHOS state; see 1ce;2ceSD;3ceU;4ceRot;5ceAma. |
| ce3U | E+SE | Electron transfer pathway (E) + S (SE) | ce and dce are in the ET-pathway state. |
| ce4Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET-pathway state. |
| ce5Ama | ROXAma | ROX |
Compare SUIT protocols
- SUIT-003 (A Advantageous alternative protocol)
MitoPedia concepts: SUIT protocol, SUIT B
