SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-: Difference between revisions
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|description=[[File:Ce_R 1S 2Omy 3U 4Rot 5Ama.jpg|right|200px]] | |description=[[File:Ce_R 1S 2Omy 3U 4Rot 5Ama.jpg|right|200px]] | ||
'''[[Categories of SUIT protocols |SUIT-catg]]:''' CC+S | '''[[Categories of SUIT protocols |SUIT-catg]]:''' CC+S | ||
|info=[[Stadlmann 2006 Cell Biochem Biophys]] | |info=[[Stadlmann 2006 Cell Biochem Biophys]] | ||
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|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol | ||
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::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | |||
::: '''[[Mark names in DatLab]]:''' Stadlmann 2006 Cell Biochem Biophys | |||
::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': Stadlmann 2006 Cell Biochem Biophys.xlsx | |||
[[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | [[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | ||
== Coupling control protocol == | == Coupling control protocol == | ||
Revision as of 14:17, 29 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
SUIT-catg: CC+S
Abbreviation: CC_R 1Omy 2U 3Rot 4S 5Ama
Reference: Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol
- SUIT protocol pattern: complex coupling control protocol (CCP)
- Mark names in DatLab: Stadlmann 2006 Cell Biochem Biophys
- DatLab-Excel template: in prep.: Stadlmann 2006 Cell Biochem Biophys.xlsx
Coupling control protocol
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| R | ROUTINE | ce | pce without substrate are in a state of ROX. |
| 1SD | R+SP | ROUTINE (R) + S (SP) | pce are in state SP. |
| 2Omy | L+SL | LEAK (L) + S (SL) | ce and pce are in the LEAK state. |
| 3U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
| 4Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
| 5Ama | ROXAma | ROX |
- R 1S .. versus R 1Omy 2U 3S .. and respiration medium
- Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
