Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
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::: '''[[SUIT protocol pattern]]:''' complex cell [[ | ::: '''[[SUIT protocol pattern]]:''' complex cell [[Coupling-control protocol]] ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama | ||
[[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | [[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | ||
[[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | [[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | ||
== Coupling control protocol == | == Coupling-control protocol == | ||
::::* 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma | ::::* 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma | ||
| Line 22: | Line 22: | ||
| ce1 | | ce1 | ||
| [[ROUTINE]] | | [[ROUTINE]] | ||
| [[ | | [[Living cells |Iiving cells (ce)]] | ||
| Dead cells (dce) without substrate are in a state of ROX. | | Dead cells (dce) without substrate are in a state of ROX. | ||
| Line 34: | Line 34: | ||
| ce3Omy | | ce3Omy | ||
| ''L''+S<sub>''L''</sub> | | ''L''+S<sub>''L''</sub> | ||
| [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | | [[LEAK respiration]] (''L'') + [[S]] (S<sub>''L''</sub>) | ||
| ce and dce are in the LEAK state. | | ce and dce are in the LEAK state. | ||
| Line 40: | Line 40: | ||
| ce4U | | ce4U | ||
| ''E''+S<sub>''E''</sub> | | ''E''+S<sub>''E''</sub> | ||
| [[ | | [[Electron transfer pathway]] (''E'') + [[S]] (S<sub>''E''</sub>) | ||
| ce and dce are in the ET state. | | ce and dce are in the ET state. | ||
| Line 59: | Line 59: | ||
:::* '''ce1;ce1S ..''' versus '''ce1;ce2Omy;ce3U;ce4S .. and respiration medium''' | :::* '''ce1;ce1S ..''' versus '''ce1;ce2Omy;ce3U;ce4S .. and respiration medium''' | ||
:::: ''' | :::: '''Living cells & succinate''': ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol, SUIT B | |mitopedia concept=SUIT protocol, SUIT B | ||
}} | }} | ||
Latest revision as of 12:33, 25 November 2020
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: FNS(Oct,PGM)
Reference: B SUIT-003 Stadlmann 2006 Cell Biochem Biophys
O2k-Application: O2
- SUIT protocol pattern: complex cell Coupling-control protocol ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama
,3L(S),4E(S)-5ROX(S),6ROX.jpg)
Coupling-control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| ce1 | ROUTINE | Iiving cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
| ce2SD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
| ce3Omy | L+SL | LEAK respiration (L) + S (SL) | ce and dce are in the LEAK state. |
| ce4U | E+SE | Electron transfer pathway (E) + S (SE) | ce and dce are in the ET state. |
| ce5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
| ce6Ama | ROXAma | ROX |
- ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium
- Living cells & succinate: ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts:
SUIT protocol,
SUIT B
