Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"

Latest revision as of 12:33, 25 November 2020

high-resolution terminology - matching measurements at high-resolution

SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-

Description

Abbreviation: FNS(Oct,PGM)

Reference: B SUIT-003 Stadlmann 2006 Cell Biochem Biophys

O2k-Application: O2

SUIT protocol pattern: complex cell Coupling-control protocol ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama

Coupling-control protocol

1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma

Step	Respiratory state	Pathway control	Comment
ce1	ROUTINE	Iiving cells (ce)	Dead cells (dce) without substrate are in a state of ROX.
ce2SD	R+S_P	ROUTINE (R) + S (S_P)	dce with functional mitochondria are in state S_P.
ce3Omy	L+S_L	LEAK respiration (L) + S (S_L)	ce and dce are in the LEAK state.
ce4U	E+S_E	Electron transfer pathway (E) + S (S_E)	ce and dce are in the ET state.
ce5Rot	ROX+S_E	ROX+S (S_E)	ce are in the ROX state, dce are in the ET state.
ce6Ama	ROX_Ama	ROX

ce1;ce1S .. versus ce1;ce2Omy;ce3U;ce4S .. and respiration medium

Living cells & succinate: ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).

MitoPedia concepts: SUIT protocol, SUIT B

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|abbr=RLE(SD)
+|abbr=FNS(Oct,PGM)
-|description=[[File:1R,2SD,3Omy,4U-5Rot,6Ama.jpg|200px]]
+|description=[[File:ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama.png|200px]]
-|info='''A''' [[Stadlmann 2006 Cell Biochem Biophys]]
+|info='''B''' '''[[SUIT-003]]''' [[Stadlmann 2006 Cell Biochem Biophys]]
+|application=O2
 }}
-{{MitoPedia concepts
+::: '''[[SUIT protocol pattern]]:''' complex cell [[Coupling-control protocol]] ce1;ce2SD;ce3Omy;ce4U;ce5Rot;ce6Ama
-|mitopedia concept=SUIT protocol, SUIT A
-}}
-::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' RLE(SD)
-::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP)
-[[File:CCP(S)02(R,L,E).jpg|right|200px]]
+[[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]]
 [[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]]
-== Coupling control protocol ==
+== Coupling-control protocol ==
-::::* 1R,2SD,3Omy,4U-5Rot,6Ama
+::::* 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
 {| class="wikitable" border="1"
@@ Line 23: / Line 20: @@
 |-
-| 1R
+| ce1
 | [[ROUTINE]]
-| [[Intact cells |ce]]
+| [[Living cells |Iiving cells (ce)]]
-| [[Permeabilized tissue or cells |pce]] without substrate are in a state of ROX.
+| Dead cells (dce) without substrate are in a state of ROX.
 |-
-| 2SD
+| ce2SD
 | ''R''+S<sub>''P''</sub>
 | [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>)
-| pce are in state S<sub>''P''</sub>.
+| dce with functional mitochondria are in state S<sub>''P''</sub>.
 |-
-| 3Omy
+| ce3Omy
 | ''L''+S<sub>''L''</sub>
-| [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>)
+| [[LEAK respiration]] (''L'') + [[S]] (S<sub>''L''</sub>)
-| ce and pce are in the LEAK state.
+| ce and dce are in the LEAK state.
 |-
-| 4U
+| ce4U
 | ''E''+S<sub>''E''</sub>
-| [[ETS]] (''E'') + [[S]] (S<sub>''E''</sub>)
+| [[Electron transfer pathway]] (''E'') + [[S]] (S<sub>''E''</sub>)
-| ce and pce are in the ETS state.
+| ce and dce are in the ET state.
 |-
-| 5Rot
+| ce5Rot
 | ROX+S<sub>''E''</sub>
 | [[ROX]]+[[S]] (S<sub>''E''</sub>)
-| ce are in the ROX state, pce are in the ETS state.
+| ce are in the ROX state, dce are in the ET state.
 |-
-| 6Ama
+| ce6Ama
 | ROX<sub>Ama</sub>
 | [[ROX]]
@@ Line 61: / Line 58: @@
-:::* '''1R 1S ..''' versus '''1R 2Omy 3U 4S .. and respiration medium'''
+:::* '''ce1;ce1S ..''' versus '''ce1;ce2Omy;ce3U;ce4S .. and respiration medium'''
-:::: '''Intact cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]).
+:::: '''Living cells & succinate''': ADP is not permeable through the plasma membrane of living cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]).
+{{MitoPedia concepts
+|mitopedia concept=SUIT protocol, SUIT B
+}}